黄芪提取物对老年痴呆大鼠海马区COX-2和iNOS表达的影响  

Effect of astragalus extract on expression of COX-2 and iNOS in the hippocampus of rats with Alzheimer disease

作　　者：单铁英[1] 高立威[2] 董洋[2] 郑秀清[3] 董静[3] 郑海萍[1] 王雪丹[1] 苏安英[1] 

机构地区：[1]河北工程大学,河北邯郸056002 [2]邯郸市中心医院,河北邯郸056002 [3]邯郸市疾病预防控制中心,河北邯郸056002

出　　处：《现代预防医学》2016年第10期1859-1861,1889,共4页Modern Preventive Medicine

摘　　要：目的研究黄芪提取物对老年性痴呆(AD)大鼠学习记忆能力的影响及其作用机制。方法 SD大鼠海马内注射Aβ25-35制备AD大鼠模型,实验分5组:正常对照组、模型组和不同浓度的黄芪组(20、40和80 mg/kg)。黄芪组在造模前后均用黄芪提取物灌胃。用Morris水迷宫测定各组大鼠的学习记忆能力,Western blot测定各组大鼠海马COX-2和i NOS蛋白表达。结果模型组的逃避潜伏期延长,单位时间内跨越原平台次数减少,海马COX-2和i NOS的表达水平升高,与正常对照组比较均有统计学差异(P<0.05);不同浓度黄芪组的逃避潜伏期均缩短,单位时间内跨越原平台次数均增多,且呈剂量依赖性;COX-2和i NOS表达水平均下降,并且随着黄芪浓度的增加COX-2和i NOS表达水平降低越明显,与模型组比较差异均有统计学意义(P<0.05)。结论经过黄芪提取物的治疗,老年痴呆大鼠的学习记忆能力得到了明显提高,其机制可能是黄芪提取物通过抑制COX-2、i NOS等信号转导途径,减轻Aβ25-35毒性作用。Objective This work was to investigate the effect of astragalus extract on learning and memory in model rats with Alzheimer disease and the related mechanisms. Methods The AD model rats were established by injecting Aβ25 - 35 into districts of hippocampuses. The rats were divided into 5 groups： control group, model group and three groups with using different astragalus concentrations （20, 40, and 80 mg/kg）. Astragalus groups were treated with extract of astragalus before and after building AD model. The ability of learning and memory was measured by Morris water maze. The protein expression level of COX - 2 and iNOS in the hippocampus of each group was measured by western blot. Results For the model group rats, the incubation period of avoiding increased, the frequency of passing through platform decreased, and the expression level of COX - 2 and iNOS increased when compared with those of normal control group, and the difference was statistically significant （P 〈 0. 05 ）. The avoiding incubation period of rats in different concentration astragalus groups was obviously shortened, the frequency of passing through platform was increased in a dose dependent manner. The expression level of COX - 2 and iNOS decreased with increasing concentration of astragalus （P 〈 0. 05 ）. Conclusions Extract of astragalus had a protective effect on learning and memory of AD rats, probably through reducing the toxic effect of Aβ25 - 35 by inhibiting signal transduction pathways of COX -2 and iNOS.

关 键 词：黄芪提取物 阿尔茨海默病 环氧化酶2 诱导型一氧化氮合酶 

分 类 号：R741.05[医药卫生—神经病学与精神病学]