喉鳞状细胞癌患者视网膜母细胞瘤蛋白和细胞周期调节蛋白D1异常表达  

The Abnormal Expressions of pRb and Cyclin D1 in Laryngeal Squamous Cell Carcinoma Patients

作　　者：曾汉荣[1] 杨虹女 李文星[2] 涂兴 李志华[2] 孔维佳[3] 

机构地区：[1]湖北省枝江市人民医院耳鼻咽喉科,枝江443200 [2]武汉大学人民医院耳鼻咽喉-头颈外科,武汉430060 [3]华中科技大学同济生命科学学院,武汉430022

出　　处：《微循环学杂志》2016年第2期66-69,共4页Chinese Journal of Microcirculation

摘　　要：目的:探讨喉鳞状细胞癌(LSCC)患者视网膜母细胞瘤蛋白(pRb)和细胞周期调节蛋白D1(Cyclin D1)的异常表达及其相关性。方法:选择36例喉部疾病手术患者,包括LSCC(LSCC组)、声带息肉(息肉组)和癌前病变(癌前组)各12例,各组均于手术后取病变组织,采用Western-blotting检测pRb和Cyclin D1蛋白表达水平,统计学分析各组两指标差异及相关性。结果:与息肉组比较,癌前组pRb表达水平降低(P<0.01),Cyclin D1表达水平升高(P<0.01);与癌前组比较,LSCC组pRb水平更低(P<0.01),Cyclin D1水平更高(P<0.01)。三组pRb与Cyclin D1蛋白水平均呈显著负相关(r分别为-0.94、-0.83和-0.92,P均<0.01)。结论:LSCC的发生和发展与pRb表达降低和Cyclin D1表达增加有关。Objective：To investigate the abnormal expressions of retinoblastoma protein （pRb） and cell cycle protein D1 （Cyclin D1） in laryngeal squamous cell carcinoma （LSCC） patients .Method：All pathological specimens , which were respectively included 12 cases of LSCC group and vocal cord polyp （VCP group） as well as laryngeal precarcinoma lesions （LPL group） were selected from patients ,who were accepted radical surgery treatments be‐cause of different diseases of laryngeal tissures .The expression of pRb and Cyclin D1 were detected by Western‐blotting methods .Moreover ,correlational difference between different proteins were analyzed and compared by used statistical software in the mentioned above three groups .Results：Compared with VCP group ,the expression of pRb was markedly decreased （P〈0 .01） ,and the expression of Cyclin D1 was notably increased in LPL group （P〈0 . 01） .Compared with LPL group ,however ,the quantitative levels of pRb was furtherly decreased （P〈0 .01） ,mo‐reover ,the quantitative levels of Cyclin D1 was significenlly increased in LSCC group （P〈0 .01） .The expression of pRb had significant negative correlation with that of Cyclin D 1 with -0 .94 in VCP group and -0 .83 in LPL group as well -0 .92 in LSCC group （P of all 〈 0 .001） .Conclusion：The occurrence and development of patients with LSCC might have significant relationship with that pRb was severely decreased and Cyclin D 1 was significantly in‐creased .

关 键 词：喉鳞状细胞癌 视网膜母细胞瘤蛋白 细胞周期调节蛋白D1 Cell CYCLE protein D1 

分 类 号：R739.65[医药卫生—肿瘤]