酶法制备多肽-Fe的体内抗氧化功能研究  

Studies on Antioxidant Effect in Vivo of Polypeptide-Fe from Hemoglobin by Enzymatic Hydrolysis

作　　者：孔宇[1] 韩冉[1] 王汝华[1] 陈文[1] 刘常金[1] 

机构地区：[1]天津科技大学食品工程与生物技术学院,天津300457

出　　处：《农产品加工》2016年第4期20-22,共3页Farm Products Processing

摘　　要：研究了猪血血红蛋白经酶解制备的多肽-Fe对小鼠体内抗氧化作用,分别测定血清中的ALT,AST,肝脏中的GSH-Px,蛋白质,SOD,MDA。结果表明,多肽-Fe可以降低由于肝脏损伤而引起的血清中AST和ALT含量的升高,同时可抑制肝脏中GSH-Px活力降低,很好地抑制小鼠肝组织中MDA的形成及肝损伤后SOD代偿性升高。HE染色结果表明,多肽-Fe可在一定程度上改善CCl4造成的肝损伤。To study the antioxidant effect in vivo of Polypeptide-Fe from Hemoglobin by enzymatic hydrolysis on mice. Contents of alanine transaminase（ALT） and aspartate transaminase（AST） in serum,and enzyme activities of glutathione peroxidase（GSH-Px） and superoxide dismutase（SOD） and malondiadehyde（MDA） and protein in liver are measured. The followings are results. Polypeptide-Fe can reduce the contents of ALT and AST which will rise after the liver damage,can inhibit the GSH-Px activity decrease,can significantly inhibit the formation of MDA in liver and the activities of SOD compensatorily increased in liver. HE dyed results show that Polypeptide-Fe has a certain improvement on liver injury.

关 键 词：猪血血红蛋白 多肽-Fe 体内抗氧化 

分 类 号：TS251.93[轻工技术与工程—农产品加工及贮藏工程]