锁阳多糖对Hela和A549细胞增殖抑制作用的比较  

Study on Inhibitory Effect of Cynomorium Polysaccharide on Hela and A549 Cells Proliferation

作　　者：张慧瑛[1] 罗光宏[2] 杨生辉[2] 郝军元[1] 崔伟[1] 

机构地区：[1]河西学院农业与生物技术学院,甘肃张掖734000 [2]河西学院凯源生物技术开发中心,甘肃张掖734000

出　　处：《河西学院学报》2016年第2期71-77,共7页Journal of Hexi University

基　　金：甘肃省青年科技研究基金项目(1107RJYG012);甘肃省科技支撑计划项目(1304FKCG103)

摘　　要：探讨锁阳多糖对人宫颈癌Hela细胞和人肺癌A549细胞的增值抑制作用,在细胞浓度为10~5个/m L中分别加入0.05、0.1、0.2、0.4和0.8mg/m L的锁阳多糖,培养120h后检测细胞生长抑制率、细胞存活率观察细胞形态的变化.结果表明：当锁阳多糖浓度为0.8mg/m L,作用时间为120h时,对Hela细胞和A549细胞的增殖抑制率分别为63.53%和67.62%,其存活率分别为37.5%和33.4%,说明锁阳多糖对Hela细胞和A549细胞均有抑制作用,且对A549细胞增殖抑制作用强于Hela细胞.To explore the inhibitory effect of Cynomorium polysaccharides on human cervical cancer cells and human lung cancer cells proliferation, in cell concentration of 105/ml were added 0.05,0.1,0.2,0.4,0.8mg/ ml Cynomorium polysaccharides, cultivate 120h,determine the cell growth inhibition rate, detect cell survival rate and observe changes in cell morphology. The results showed that： the cell growth inhibition rates of Hela and A549 cells were 63.53% and 67.62% for Cynomorium polysaccharide with series concentration of 0.8 rag/ mL,action time is 120h. the survival ates were 37.5% and 33.4%.Indicating that Cynomorium polysaccharides extract have inhibiting effect to Hela and A549 cells and inhibition effect on A549 cells was stronger than Hela ceils in vitro cultured.

关 键 词：锁阳多糖 MTT法 HELA细胞 A549细胞 抑制作用 

分 类 号：TS201.2[轻工技术与工程—食品科学]