猪瘟疫苗冻干保护剂的筛选及免疫效果评价  

Screening of freeze-drying protectants of swine fever virus vaccine and effect evaluation for oral immunization

作　　者：李星[1,2] 陶洁[2,3] 殷秀辰[2,3] 张清真[1,2] 熊年年 刘惠莉[2,3] 

机构地区：[1]上海海洋大学水产与生命学院,上海201306 [2]上海市农业科学院畜牧兽医研究所,上海201106 [3]上海市农业遗传育种重点实验室,上海201106

出　　处：《上海农业学报》2016年第2期19-23,共5页Acta Agriculturae Shanghai

基　　金：上海市科技兴农重点攻关项目[沪农科攻字(2013)第3-6号;沪农科攻字(2010)第(12-1)号];上海市生猪产业体系动物疫病防控技术组岗位专家项目

摘　　要：为了筛选耐热性能好、能提高猪瘟病毒(SFV)疫苗经口服途径免疫效果的冻干保护剂,配比不同组分的冻干保护剂与黏膜佐剂LTRG蛋白、SFV按比例混合,通过对冻干工艺的优化,制备了4种.SFV的冻干苗;口服免疫小鼠,测定血清IgG和粘膜IgA抗体效价。结果表明:试验2组即甘氨酸+明胶组和试验4组即明胶组的外观形态较好,水溶后呈现澄清的状态;试验2组和试验4组的冻干苗经口服免疫,诱导小鼠产生的IgG抗体水平显著高于SFV组、SFV+LT组及其他试验组(P<0.01)。二免7 d试验2组小鼠肛试子IgA抗体显著高于其他组(P<0.01),且小鼠脾脏IFN-γ和IL-4细胞因子的表达量也极显著高于其他组(P<0.01)。研究初步筛选出第2组配方,即以甘氨酸和明胶配伍作为猪瘟口服疫苗冻干保护剂,为研制口服途径免疫的猪瘟疫苗奠定了基础。In order to select a freeze-drying protectant having a good heat tolerance and improving oral im- munization of swine fever virus （ SFV ） vaccine, freeze-drying proteetants with different components were mixed with mucosal adjuvant LTRG protein and SFV in proportion, and 4 kinds of lyophilized SFV vaccines were pre- pared via optimization of freeze-drying conditions;The vaccines were used for oral immunization of mice and the serum IgG and mucosal IgA antibody valences were determined. The results showed that Group 2 （ glycine ＋ ge- latin） and Group 4 （gelatin）had a relatively good appearance and water solubility;After oral immunization the mice-produced IgG antibody levels in Groups 2 and 4 were very significantly higher than those in other groups, and at 7 days after the second oral immunization the anal swab＇ s IgA antibody level in Group 2 was very signifi- cantly higher than that in other groups, and moreover, the spleens＇ IFN-γ and IL-4 cytokine expression levels in Group 2 were very significantly higher than those in other groups. It was concluded that gelatin and glyeine could be used as freeze-drying protectants of oral SFV vaccine.

关 键 词：猪瘟疫苗 黏膜佐剂LTRG蛋白 冻干保护剂 口服免疫 效果评价 

分 类 号：S858.28[农业科学—临床兽医学]