水通道蛋白3对胃癌细胞株SGC7901中基质金属蛋白酶的调节作用  

Aquaporin-3 pathways regulated matrixmetalloproteinase expression in human gastric carcinoma SGC-7901 cells

作　　者：徐勇[1] 李忠[1] 王勃[1] 董雪伟[1] 卢麒丞[1] 

机构地区：[1]常州市第一人民医院普外科,江苏常州213003

出　　处：《常州实用医学》2016年第2期71-74,共4页CHANGZHOU PRACTICAL MEDICINE

摘　　要：目的探讨水通道蛋白3对胃癌细胞株SGC7901中基质金属蛋白酶的调节作用。方法构建水通道蛋白3的过表达及干扰质粒，由慢病毒作载体分别转染SGC7901细胞，随后检测SGC7901细胞中MMP表达的变化。结果SGC7901细胞的AQP3被敲除后，MTl-MMP、MMP-2、MMP-9的表达显著降低，而当其中AQP3过表达时，MTl-MMP、MMP-2、MMP-9的表达又明显增加；SGC7901细胞中AQP3的沉默可导致AKT途径ser473磷酸化水平降低。结论人胃癌细胞SGC7901中水通道蛋白3经P13K／AKT途径正向调节基质金属蛋白酶的表达。Objectives To investigate the role of water channel protein 3 in the regulation of matrix metal- loproteinases in human gastric cancer cell line SGC7901. Methods In this study, AQP3 was silenced or over- expressed in SGC7901 cells. Results We found a significant decrease in MT1-MMP, MMP-2, and MMP-9 ex- pression after AQP3 knockdown, and a significant increase in MT1-MMP, MMP-2, and MMP-9 expression after AQP3 over-expression in SGC7901 cells. We also found that AQP3 silence led to a significant decrease of phos- phorylation of set473 in AKT in SGC7901 cells. Conclusion Our findings showed that AQP3 might positively reg- ulate MMPs proteins expression through PI3IGAKT signal pathway in human gastric carcinoma SGC7901 cells.

关 键 词：水通道蛋白3 基质金属蛋白酶 P13K/AKT通路 

分 类 号：R73-3[医药卫生—肿瘤]