不同浓度尿酸对体外红细胞流变学指标的影响  

Effects of different concentrations of uric acid on erythrocyte hemorheology in vitro

作　　者：李葵花[1] 李璐[2] 严鹏[1] 佟晓波[1] 谢利德[1] 

机构地区：[1]承德医学院生物医学工程系,河北承德067000 [2]河北大学附属医院麻醉科,河北保定071000

出　　处：《河北大学学报（自然科学版）》2016年第2期184-188,共5页Journal of Hebei University(Natural Science Edition)

基　　金：河北省高等学校科学技术研究项目(QN20131033);承德医学院自然科学一般项目(201313);承德医学院大学生科研立项(201439)

摘　　要：探讨了不同浓度尿酸水平对体外红细胞流变学指标的影响.300、600、1 200μmol/L的尿酸对体外红细胞作用2h后,测量红细胞的流变学指标,并应用SDS-PAGE对红细胞膜蛋白成分进行分析.随尿酸浓度的增加,体外红细胞的积分变形指数、松弛指数降低,红细胞的破碎率升高.与对照组相比,红细胞锚蛋白及带3蛋白含量减少,1 200μmol/L尿酸组的相关指标具有明显差别.提示高浓度尿酸可对红细胞膜蛋白产生明显的破坏作用,是引起红细胞流变学特性改变的独立因素.The effects of different concentrations of uric acid on hemorheology were investigated invitro. The erythrocytes were suspended in 300,600,1 200/lmol/L uric acid for 2 h and the hemorheologicalparameters were measured. The membrane protein composition was also analyzed by SDS-PAGE. We foundthat the integrated deformation index and relax index were reduced in a dose-dependent manner. The he-molysis rate increased. As compared with the control group, the content of ankyrin and band 3 proteinsshowed a decrease trend. These results were statistically significant in the 1 200μmol/L group. The resultssuggest that high concentrations of uric acid can have significant damaging effects on erythrocyte mem-brane proteins,which is an independent factor affecting the hemorheological properties of erythrocytes.

关 键 词：尿酸 红细胞 血液流变学 膜蛋白 

分 类 号：R35[医药卫生—基础医学]