S100钙结合蛋白A6在胃癌组织中表达的意义  

S100 calcium binding protein A6 expression and significance in gastric cancer

作　　者：马亮[1] 赵滢[1] 鲍晨辉[1] 陈鑫莹[1] 

机构地区：[1]中国医科大学附属盛京医院胃肠营养外科,辽宁沈阳110004

出　　处：《临床军医杂志》2016年第4期423-426,共4页Clinical Journal of Medical Officers

基　　金：辽宁省科技厅科学计划项目(2013225021)

摘　　要：目的探讨S100钙结合蛋白A6(S100A6)在胃癌旁正常组织、慢性胃炎、肠上皮化生和胃癌的组织标本中的表达,以及其与胃癌临床病理特征及预后的关系。方法收集中国医科大学附属盛京医院2005年6月至2015年6月胃组织标本,其中胃黏膜肠上皮化生组织(24例)、胃癌(42例)及癌旁正常组织标本(42例)。qRT-PCR法检测S100A6 mRNA在不同组织中表达;Western blot法检测S100A6蛋白的表达;采用Kaplan-Meier生存曲线用于区分和比较生存率;Cox比例风险模型用于多因素分析。结果 S100A6蛋白的表达按以下顺序逐渐增加:癌旁正常组织<慢性胃炎<肠上皮化生<胃癌。S100A6蛋白的表达与胃癌肿瘤大小、浸润深度、淋巴管和静脉侵犯、淋巴结转移、肿瘤TNM分期呈正相关。Kaplan-Meier生存曲线提示,高中度S100A6基因表达的患者累积生存率明显低于不表达的患者。Cox比例风险模型提示肿瘤的浸润深度、淋巴管及静脉浸润、淋巴结转移、TNM分期以及S100A6高表达均为胃癌患者预后的独立因素。结论在胃炎、肠上皮化生、胃癌中S100A6蛋白的表达逐渐升高;S100A6高阳性表达是胃癌预后的独立影响因素。Objective To investigate the relationships among S100A6′s（S100 calcium-binding protein A6）expression in normal tis-sue samples,chronic gastritis,intestinal metaplasia and gastric cancer,clinical characteristics and prognosis. Methods The stomach tissue samples were collected in our hospital from June 2005 to June 2015,Gastric mucosa of intestinal metaplasia group（24 cases）, gastric cancer（42 cases）and normal tissue adjacent to carcinoma specimens（42 cases）. HE staining use of gastric specimens was taken for histopathological diagnosis;using immunohistochemistry to detect the expression of tissue S100A6 protein;qRT-PCR was used to detect mRNA expression of the S100A6 gene in different tissue samples;Western Blot detection of S100A6 gene encoding protein. Kaplan-Meier survival curves were used to distinguish and compare survival. Cox proportional hazards model for multiple fac-tors analysis. Results S100A6 protein and mRNA expression was gradually increased in the following order：normal tissue〈gastritis〈intestinal metaplasia〈carcinoma. S100A6 levels with tumor size,depth of invasion,lymphatic invasion,lymph node metastasis and lymph node metastasis （ TNM ） staging;however, with cancer patients independent of age and gender. Kaplan-Meier survival curves showed that patients who had high expression of S100A6 gene expressed had lower survival rates compared with others. Cox proportion-al hazards models suggested tumor invasion depth,lymphatic and venous invasion,lymph node metastasis,TNM staging and according to the A6 high expression were independent factors of prognosis of patients with gastric cancer. Conclusion The expression of S100A6 in carcinoma was higher than that in normal tissue and gastritis. Thus S100A6 gene may play an important role in the malignant trans-formation of epithelial cells in the stomach which was an independent prognostic factor to provide a basis for clinic treatment and prog-nosis evaluation.

关 键 词：S100钙结合蛋白A6 胃癌 免疫组化 RT-PCR WESTERN BLOT法 

分 类 号：R735.2[医药卫生—肿瘤]