贝美格致惊大鼠海马凋亡抑制基因bcl-2表达年龄特征  

AGE CHARACTERISTICS OF ANTI-APOPTOTIC GENE BCL-2 EXPRESSION IN HIPPOCAMPUS OF RATS AFTER CONVULSION INDUCED BY 3-ETHYL-3-METHYL GLUTARIMIDE

作　　者：苑爱云[1] 张雷红[1] 刘秋燕[1] 侯梅[1] 

机构地区：[1]青岛大学医学院附属妇女儿童医院神经康复科,山东青岛266034

出　　处：《齐鲁医学杂志》2016年第2期177-180,共4页Medical Journal of Qilu

基　　金：山东省医药卫生科技发展计划项目(2015WS0352)

摘　　要：目的探讨3-乙基-3-甲基戊二酰亚胺（贝美格）致惊后大鼠海马凋亡抑制基因bcl-2表达的年龄特征及变化规律。方法取健康成年和幼年Wistar大鼠,经腹腔注射贝美格制作长时程惊厥模型,惊厥持续15min后止惊。成年和幼年两组大鼠分别于惊厥后3h、6h、1d、3d、7d断头处死,用免疫组化、原位杂交及逆转录聚合酶链反应方法检测海马bcl-2蛋白及mRNA表达的动态变化。结果惊厥后3、6h,两组大鼠海马bcl-2蛋白表达较相应正常对照显著升高（F=7 484.0、9 599.0,t=118.9~155.3,P〈0.01）。两组大鼠海马bcl-2蛋白的表达均于惊厥后6h达高峰,随后成年鼠bcl-2蛋白表达回落迅速,惊厥后1d即降至正常对照水平,而幼年鼠回落缓慢,在惊厥后3d内呈持续强表达,直至惊厥后7d仍显著高于正常对照（t=3.1,P〈0.05）。惊厥后幼年鼠bcl-2蛋白表达较成年鼠有增高趋势,在惊厥后6h及1d时两组差异有统计学意义（t=39.4、104.4,P〈0.01）。惊厥后两组大鼠海马bcl-2mRNA的表达与其蛋白表达规律类似。结论长时程惊厥可诱导海马凋亡抑制基因bcl-2表达增高,但存在年龄差异,幼年鼠bcl-2的表达强度及持续时间明显强于成年鼠,这可能是未成熟脑耐受惊厥性脑损伤的机制之一。Objective To explore the age characteristics and change rule of anti-apoptotic gene bcl-2 expression in the hippocampus of rats after convulsion induced by 3-ethyl-3-methyl glutarimide. Methods A long-term convulsion model was created in young and adult Wistar rats by intraperitoneal injection of 3-ethyl-3-methyl glutarimide. Convulsion lasted for 15 mi- nutes. The experimental rats were sacrificed-3 hours,6 hours, 1 day and 3 days, respectively, after convulsion. Dynamic changes of bcI-2 protein and mRNA levels were measured using immunohistochemistry, in-situ-hybridization and RT-PCR. Results Three and 6 hours after the onset of convulsion, the expression of hcl-2 protein in adult rats and young rats was higher than that in the controls. The expression of bcl-2 protein in young and adult rats reached its peak 6 hours after the onset of convulsion, increased in both IRs and ARs, and presented a dynamic changing process. Bcl-2 protein levels increased significantly at 3 h and 6 h and reached its peak at 6 h after the onset of convulsion in both young and adult rats, and then the expression of bcl-2 protein in the adult rats fell down quickly, and returned to normal level in rats of the controls in one day, and that in young rats dropped slowly, the ex- pression still showed hyper-expression within three days, and up to seven days after the convulsion, the expression remained higher than that in the control group （t = 3.1, P 〈 0.05）. After convulsion, the expression of bcl-2 protein showed increasing trend in young rats versus adult ones, the differences of the expression in 6 hours and one day after the onset of convulsion between the aldult and young ones were significant （t = 39.4,104.4 ; P〈 0.01）. After the onset of convulsion, the expression of bcl-2 mRNA and its law of protein expression were similar. Conclusion Long-term convulsion may strengthen the expression of anti-apoptotic gene bcl-2 in the hippocampus, but there is difference in age. The strength and persistent period in young rats are st

关 键 词：贝美格 惊厥 基因 BCL-2 大鼠 海马 年龄因素 

分 类 号：R742.1[医药卫生—神经病学与精神病学]