肠道病毒性脑炎的患儿T细胞活化亚群和细胞因子的变化  

The T cell activation and cell factor＇s changes in enterovirus encephalitis children

作　　者：方春艳[1,3] 邹小杰[2] 袁哲峰[1] 黄明海[3] 高峰[1] 

机构地区：[1]浙江大学医学院附属儿童医院,杭州310000 [2]浙江省湖州市中心医院,湖州313000 [3]宁波市妇女儿童医院,315000

出　　处：《中华实验和临床病毒学杂志》2016年第2期171-174,共4页Chinese Journal of Experimental and Clinical Virology

摘　　要：目的 通过对肠道病毒性脑炎（Enterovirus Encephalitis,EVE）的患儿T细胞活化亚群、细胞因子的检测,探讨其在EVE发病机制中的可能作用.方法 选择2014年6月至2015年7月浙江大学医学院附属儿童医院神经科病房收住院的24例脑脊液肠道病毒阳性的EVE患儿为病例组,其中14例予活化T细胞亚群检测,24例均予细胞因子流式测定,同期门诊体检的49例健康儿童为对照组.通过流式细胞术检测两组外周血CD25 ＋/CD3＋ CD4＋、CD69 ＋/CD3＋CD4＋、HLA-DR/CD3＋ CD4＋、CD25 ＋/CD3＋ CD8＋、CD69 ＋/CD3＋ CD8＋、HLA-DR/CD3＋ CD8＋,及IL-2、IL-4、IL-6、IL-10、TNF-α、INF-γ水平.结果 病例组外周血CD69/CD3＋ CD4＋、CD69/CD3＋ CD8＋的表达含量（1.193％ ±2.849％,0.696％±1.242％）低于对照组（Z=-2.722,P=0.006;Z=-3.321,P=0.001）.外周血病例组IL-2的表达水平（3.342±1.089 pg/ml）低于对照组（Z=-2.722,P=0.019）;而IL-6,TNF-α的表达水平（123.046±355.349;3.367±1.717 pg/ml）,与对照组相比,有所增加,差异有统计学意义（Z=-3.471,P=0.001;Z=-4.803,P＜0.001）.结论 EVE患儿存在一定程度的免疫功能紊乱,T细胞活化亚群和细胞因子可能参与了EVE的发病过程.Objective To explore the action of immune function in Enterovirus Encephalitis （EVE） children by detecting the T cell activation and cell factors.Methods 24 infant patients with EVE hospitalized in the wards of department of neurology of the Children＆#39; s Hospital Affiliated to Medical School of Zhejiang University from June 2014 to July 2015 were selected as the experimental group,14 of the 24 patients were detected for the T cell activation.While another 49 healthy infants accepting physical check in the same period were selected as the control group.Flow cytometry was adopted to detect the peripheral blood CD25 ＋/CD3 ＋ CD4 ＋,CD69 ＋/CD3 ＋ CD4 ＋,HLA-DR ＋/CD3 ＋ CD4 ＋,CD25 ＋/CD3 ＋ CD8 ＋,CD69 ＋/CD3 ＋ CD8 ＋,HLA-DR ＋/CD3 ＋ CD8 ＋,and IL-2,IL-4,IL-6,IL-10,TNF-α,IFN-γ levels of the two groups.Results The peripheral blood CD69/CD3 ＋ CD4 ＋、CD69/CD3 ＋ CD8 ＋ expression levels （1.193% ± 2.849%,0.696% ± 1.242%） of the experiment group were lower than those of the control group （Z =-2.722,P=0.006;Z=-3.321,P=0.001）.The expression level of the peripheral blood IL-2 （3.342 ± 1.089 pg/ml） was lower than that of the control group（Z =-2.722,P =0.019）;while the expression of IL-6 and TNF-α （123.046 ± 355.349;3.367 ± 1.717pg/ml） were significantly higher than the control group （Z =-3.471,P =0.001;Z =-4.803,P 〈 0.001） . Conclusion There is certain degree of immune dysfunction in EVE patients,T cell activation subgroup and the cell factors may participate in the morbidity progress of EVE.

关 键 词：肠道病毒性脑炎 脑脊液肠道病毒DNA阳性 T-淋巴细胞 细胞因子类 

分 类 号：R725.1[医药卫生—儿科]