PDTC对MG63细胞中周期蛋白D1及β-catenin蛋白表达影响的研究  

Effects of Expression of CyclinD1 and β-catenin Induced by PDTC in MG63 Cell

作　　者：王世振[1] 马宁[1] 窦林波[1] 矫国田[1] 胡庆龄[1] 周军[1] 卢春英[1] 

机构地区：[1]德州市人民医院口腔科,山东德州253000

出　　处：《德州学院学报》2016年第2期74-77,共4页Journal of Dezhou University

摘　　要：了解NF-κB和Wnt/β-catenin两条通路之间是否有诱导或抑制关系,从而明确成骨细胞增殖的生物学机制.以二硫代氨基甲酸吡咯烷(PDTC 0、100、200、400μmol/L)干预MG63细胞,在CO2恒温孵育箱中培养,采用Wsetern Blot方法,检测在不同时间点(12、24、48h)MG63细胞中β-catenin和CyclinD1蛋白的表达.结果表明随着浓度的增加,MG63细胞中β-catenin蛋白的表达量增高且CyclinD1蛋白的表达呈现先增高后降低的趋势,在24小时蛋白表达达到顶峰.从而得知,MG63细胞中NF-κB与Wnt/β-catenin通路之间存在相互作用,共同调节着CyclinD1的表达.By understanding the relationship between induction and inhibition of NF-κB and Wnt/β-catenin,and we can determine the biological mechanisms of bone cell proliferation.Different concentration（0,100,200,400μmol/L）PDTC were added to MG63 cells then were incubated in CO2 thermotank.Western-blot was used to detect the expression ofβ-catenin and CyclinD1.Results：As is showen in Western-blot,the expression ofβ-catenin present obvious increase with PDTC concentration increasing,and CyclinD1 proteins present obvious increasing trend in the early time,then had a decreased trend from24 hours.The＂interaction＂between NF-κB and Wnt pathways in MG63 cells involved in the expression of CyclinD1.

关 键 词：二硫代氨基甲酸吡咯烷 MG63细胞 Β-连环蛋白 周期蛋白D1 

分 类 号：R783.4[医药卫生—口腔医学]