山竹果皮提取物对人肝癌细胞HepG2增殖及凋亡的影响  

Effects of mangosteen extract on proliferation and apoptosis of human hepatocellular carcinoma HepG2 cells

作　　者：赖燕燕[1] 黄应雯 黄丹玚 伍兰岚 罗彬尤 李晓龙[3] 

机构地区：[1]广西卫生职业技术学院细胞生物学与遗传学教研室,南宁市530021 [2]广西中医药大学第一附院医院办公室,南宁市530021 [3]广西医科大学,南宁市530021

出　　处：《广西医学》2016年第4期452-456,共5页Guangxi Medical Journal

基　　金：广西医科大学青年科学基金项目(GXMUYSF09);实验室开放课题(02610212063)

摘　　要：目的探讨山竹提取物对人肝癌细胞HepG2增殖及凋亡的影响及其作用机制。方法采用MTT法检测不同浓度山竹提取物对HepG2细胞增殖的影响;分别应用Annexin-V/PI双重染色、PI单染法检测山竹提取物对HepG2凋亡和细胞周期的影响;天冬氨酸特异性半胱氨酸蛋白酶-3(Caspase-3)试剂盒检测山竹提取物对HepG2细胞Caspase-3酶活化的影响。结果不同浓度(100μmol/L、200μmol/L、400μmol/L、600μmol/L、800μmol/L)山竹提取物均可抑制人肝癌细胞HepG2的增殖活性,且随着山竹提取物浓度及其作用时间的增加,细胞抑制率升高(P<0.05);山竹提取物浓度为256.67μmol/L时可诱导人肝癌细胞HepG2出现早期凋亡,并且随着山竹提取物作用时间的增加,凋亡早期癌细胞的比率增高。细胞周期分析结果显示随着山竹提取物的浓度升高(0μmol/L、200μmol/L、400μmol/L、600μmol/L、800μmol/L),G1期HepG2细胞比例升高,而S期细胞比例下降(P<0.05)。与对照组(0μmol/L)比较,各浓度(200μmol/L、400μmol/L、600μmol/L、800μmol/L)山竹提取物组HepG2细胞的Caspase-3酶活性均升高(P<0.05),给药组的酶活力单位随给药浓度的增加而明显增加(P<0.05)。结论山竹提取物对人肝癌细胞HepG2有抑制增殖和促进凋亡的作用,其作用机制可能与抑制HepG2细胞进入S期和激活Caspase-3有关。A Objective To explore the effects of mangosteen extract on the proliferation and apoptosis of human hepatocellular carcinoma HepG2 cells and its mechanism. Methods Methylthiazolyl tetrazolium（MTF） assay was used to assess the effect of mangosteen extract on the proliferation and apoptosis of HepG2 cells with various concentrations. Annexin-V/PI double staining method and PI single staining method were used to determine the effect of mangosteen extract on the apoptosis and cell cycle of HepG2 cells respectively. Cysteine-containing aspartate-specific proteases-3 （Caspase-3） kit was used to assess the effect of mangosteen extract on the Caspase-3 activation of HepG2 cells. Results The mangosteen extract with various concentrations（ 100 μmol/L,200 p, mol/L,400 μmol/L,600 μmol/L and 800 panol/L） could inhibit the proliferative activity of HepG2 cells, and the inhibitory rate of the cells increased with the increasing concentration of drug and duration of drug action （ P 〈 0.05 ）. The mangosteen extract with the concentration of 256.67 μmol/L could induce early apoptosis in HepG2 cells, and the ratio of the ceils in early apoptotic stage increased significantly with the increasing duration of drug action. Cell-cycle analysis revealed that the ratio of HepG2 cells in the G1 phase increased and the ratio of the cells in the S phase reduced with the increasing mangosteen extract concentration（0 μmol/L,200 μmol/L,400 pjnol/L,600 μmol/L and 800 μmol/L） （P 〈0.05）. Compared to the control group （0μmol/L） ,the Caspase-3 activities of HepG2 cells in the groups with different mangosteen extract concentrations（200 μmol/L,400 μmol/L, 600 μmol/L and 800 μmol/L） increased（P 〈0. 05）,and the Caspase-3 activities of HepG2 ceils increased with the increasing mangosteen extract concentration（P 〈 0.05 ）. Conclusion Mangosteen extract can inhibit the proliferation and promote the apoptosis of human hepatocellular carcinoma HepG2 cells. The mechanism may be related to the inhibi

关 键 词：肝癌 人肝癌细胞HEPG2 山竹提取物 增殖 凋亡 细胞周期 天冬氨酸特异性半胱氨酸蛋白酶-3 

分 类 号：R735.7[医药卫生—肿瘤]