甲胎蛋白阴性与甲胎蛋白阳性肝细胞癌基因表达谱差异研究  

Differences in mRNA expression profiles between alpha-fetoprotein negative and positive hepatocellular carcinoma

作　　者：安松林[1] 肖汀[2] 冯林[2] 郭宏林[2] 荣维淇[3] 王黎明[3] 吴凡[3] 冯莉[3] 吴健雄[3] 

机构地区：[1]首都医科大学附属北京世纪坛医院肿瘤外二科,北京100038 [2]北京协和医学院中国医学科学院肿瘤医院分子肿瘤学国家重点实验室 [3]北京协和医学院中国医学科学院肿瘤医院腹部外科

出　　处：《中华普通外科杂志》2016年第5期411-414,共4页Chinese Journal of General Surgery

基　　金：北京协和医学院协和青年基金、中央高校基本科研业务费专项资金资助项目（33320140163）

摘　　要：目的肝细胞癌是具有不同临床表现和分子生物学特征的异质性疾病，本研究比较甲胎蛋白（alpha—fetoprotein，AFP）阴性和AFP阳性肝癌的mRNA表达谱差异，并进行生物信息学分析。方法采用安捷伦4×44k基因芯片平台，检测合并乙型肝炎病毒（hepatitis Bvirus，HBV）感染的20例AFP阴性和20例AFP阳性肝癌组织的mRNA表达谱，用生物信息学方法进行差异基因分析及GOPathway分析，并用realtimePCR验证芯片结果。结果通过表达谱芯片分析，在AFP阴性和AFP阳性肝癌组织中发现352个差异表达基因。主成分分析及GOPathway分析显示，AFP阴性肝癌组织高表达基因62个，主要参与细胞凋亡和程序性死亡等过程，AFP阳性肝癌高表达基因290个，主要参与有丝分裂和细胞周期等过程。realtimePCR验证差异基因LAMB3和STARD5的表达．结果与表达谱芯片结果一致。结论AFP阴性肝癌和AFP阳性肝癌基因表达特征不同。提示不同AFP水平的肝癌的发生和发展存在不同的分子机制。Objective To compare gene expression profiles between AFP negative and AFP positive hepatocellular carcinoma （HCC）. Methods The analysis of mRNA expression was performed on 20 samples of AFP negative and 20 samples of AFP positive HCC associated with HBV infection through Agilent Whole Human Genome Oligo Microarray. Bioinformatics analyses were used to identify genes and real time PCR was applied to verify microarray data. Results 352 differentially expressed genes in the two groups were identified through principal component analysis. 62 up-regulated genes in AFP negative HCC were found to be involved in cell apoptosis and programmed cell death, whereas 290 up-regulated genes in AFP positive HCC involved in mitosis and cell cycle. Two differentially expressed genes including LAMB3 and STARD5 were further tested through real time PCR, and the results were in high concordance with microarray resnhs. Conclusion The gene expression profiles of AFP negative HCC were different from AFP positive HCC. This study suggests there are different molecular mechanisms in HCC development and progression with different AFP levels.

关 键 词：癌 肝细胞 甲胎蛋白类 

分 类 号：R735.7[医药卫生—肿瘤]