DEK表达下调对胃癌SGC-7901细胞增殖和侵袭的影响  

Effects of DEK downregulation on proliferation, cell cycle and invasion ability of gastric carcinoma SGC-7901 cells

作　　者：张彩凤[1] 董良鹏[2] 李少华[3] 王加一[3] 郭晓鹤[1] 张利利[1] 王现兵[3] 贾振飞[3] 周慧聪[1] 

机构地区：[1]新乡医学院第一附属医院消化科,河南省453100 [2]新乡医学院第一附属医院普通外科,河南省453100 [3]安阳市人民医院普通外科

出　　处：《中华普通外科杂志》2016年第5期415-418,共4页Chinese Journal of General Surgery

基　　金：河南省卫生厅科技攻关资助项目（200804055）;新乡医学院科研培育基金资助项目（2013QN128）

摘　　要：目的探讨DEK表达下调对胃癌细胞增殖、周期和侵袭能力的影响。方法将DEKsiRNA和对照siRNA转染胃癌SGC-7901细胞，并将SGC-7901细胞分为3组：未处理组、对照siRNA及DEKsiRNA组。采用Westernblotting技术检测3组SGC．7901细胞中DEK蛋白的表达．利用CCK．8试剂检测3组SGC-7901细胞的增殖能力，采用流式细胞术和Boyden小室检测3组SGC-7901细胞周期的分布和侵袭能力的变化，采用Westernblotting技术检测3组SGC．7901细胞中p21、cyclinD1、周期素依赖性激酶2、基质金属蛋白酶2 （ matrix metalloproteinase2, MMP-2 ）和MMPO蛋白表达的变化。结果与未处理组（0．671±0．033）和对照siRNA组（0．658±0．027）相比，DEKsiRNA组中DEK蛋白的表达水平（0．204±0．027）下调（P〈0．05）。DEK表达下调抑制胃癌SGC-7901细胞的增殖、改变细胞周期分布和降低细胞的侵袭能力。DEK表达下调能引起cyclin D1、周期素依赖性激酶2、MMP-2和MMP-9蛋白表达的下调，而p21蛋白表达上调。结论DEK表达下调介导的胃癌生物学行为的变化可能与细胞周期和侵袭相关蛋白表达的变化密切相关。Objective To explore the effects of DEK downregulation on cell proliferation, cell cycle and invasion ability of gastric carcinoma, and to investigate their possible molecular mechanisms. Methods DEK siRNA and control siRNA were used to transfect into gastric carcinoma SGC-7901 ceils, and SGC-7901 cells with different treatments were divided into three groups： untreated group, control siRNA group and DEK siRNA group. DEK protein expression was detected by Western blotting in differently treated SGC-7901 cells. Changes of cell proliferation were examined by CCK-8 kit, cell cycle distribution and cell invasion detected by flow cytometry and Boyden chamber. Expressions of p21, cyclin D1, CDK2, MMP-2 and MMP- 9 proteins were detected by Western blotting. Results Compared with untreated group （0. 671±0. 033） and control siRNA group （0. 658±0. 027 ）, expression of DEK protein was downregulated in DEK siRNA group （ 0. 204±0. 027 ） （ P 〈 0. 05 ）. DEK downregulation suppressed the proliferation, altered the distribution of cell cycle and reduced cell invasion ability in gastric carcinoma SGC-7901 cells. DEK downregulation triggered the decreases of cyclin D1, CDK2, MMP-2 and MMP-9 proteins as well as the increase of p21. Conclusions DEK downregulation mediated changes of biological behaviors in gastric carcinoma may be associated with the changes of cell cycle and invasion related proteins.

关 键 词：胃肿瘤 细胞增殖 细胞周期 肿瘤侵润 

分 类 号：R735.2[医药卫生—肿瘤]