E盒锌指蛋白1反转录本1对人膀胱癌细胞增殖、迁移及凋亡的影响  

Effects of ZEB1-AS1 on proliferation,migration and apoptosis of bladder cancer cells

作　　者：王剑锋[1] 李平[1] 

机构地区：[1]安徽医科大学附属安徽省立医院,合肥230001

出　　处：《山东医药》2016年第18期15-17,共3页Shandong Medical Journal

基　　金：国家中医药管理局中医肿瘤病学重点学科经费资助[国中医药发(2009)30号]

摘　　要：目的研究E盒锌指蛋白1反转录本1(ZEB1-AS1)对人膀胱癌细胞5637增殖、迁移、凋亡的影响。方法取适量对数生长期5637细胞分为观察组和对照组,分别用特异性的阳性对照小干扰RNA ZEB1-AS1、特异的阴性对照小干扰RNA转染细胞,采用实时荧光定量qRT-PCR法观察转染48 h两组细胞ZEB1-AS1 mRNA;采用MTT法观察转染24、48、72 h细胞增殖情况,采用细胞划痕实验观察转染48 h细胞迁移情况,采用流式细胞仪检测转染48 h细胞凋亡情况。结果转染48 h观察组和对照组ZEB1-AS1 mRNA的相对表达量分别为0.525 3±0.043 2、1.000 0±0.020 2,P<0.05;观察组转染24、48、72 h细胞增殖的OD值分别为0.316 4±0.007 8、0.595 4±0.032 5、0.770 3±0.030 7,对照组分别为0.452 9±0.016 1、0.732 6±0.037 0、0.920 4±0.018 9,组间比较P均<0.05;转染48 h时观察组和对照组的细胞迁移距离分别为(0.435±0.005)、(0.680±0.020)cm,P<0.05。转染后48 h观察组和对照组的细胞凋亡指数分别为15.70±0.94、14.53±0.69,P<0.05。结论 ZEB1-AS1可促进人膀胱癌细胞增殖、迁移,抑制细胞凋亡,可能在膀胱癌的发生、发展中起重要作用。Objective To study the effects of zinc E box 1 antisense 1 （ ZEB1-AS1 ） on proliferation, migration and apoptosis of human bladder cancer 5637 cell line （5637 for short as follow）. Methods We took suitable amount of 5637 cells in logarithmic phase and divided them into the observation group and control group, which were respectively transfect- ed with the specific positive control small interference RNA ZEB1-AS1 （ si-ZEB1-AS1 ） and the specific negative control small interference RNA （si-NC）. The real-time qRT-PCR was used to detect ZEB1-AS1 mRNA after 48-hour transfection. MTr assay was used to detect the cell proliferation at 24, 48, 72 h after transfection, scratch assay was used to detect the cell migration at 48 h after transfection, and flow cytometry was used to detect the apoptosis at 48 h after transfection. Re- suits After 48-hour transfection, the relative expression of ZEB1-AS1 mRNA in the observation group and control group was respectively 0.525 3 ± 0. 043 2 and 1. 000 0 ± 0.020 2, P 〈 0. 05 ; the OD values of the observation group at 24, 48 and 72 h after transfection were respectively 0. 316 4 ±0. 007 8, 0. 595 4 ±0.032 5 and 0.770 3 ±0.030 7, and those in the control group were respectively 0. 452 9 _± 0.016 1,0. 732 6 ± 0.037 0 and 0.920 4 ± 0.018 9, all P 〈 0.05. The cell migration distance after 48-hour transfection in the observation group and control group were respectively （0.435 ± 0.005） and （0.680 ± O. 020） cm, P 〈 0.05. After 48-hour transfection, the cell apoptosis index in the observation group and con- trol group were respectively 15.70 ± 0.94 and 14.53 ± 0.69, P 〈 0.05. Conclusion ZEB1-AS1 can promote the cell pro- liferation, migration, and inhibit apoptosis of human urinary bladder cancer ceils, which may play an important role in the occurrence and development of bladder cancer.

关 键 词：膀胱癌 长链非编码RNA E盒锌指蛋白1反转录本1 细胞增殖 细胞凋亡 细胞迁移 

分 类 号：R737.14[医药卫生—肿瘤]