snoRNA表达谱与胃癌预后的关联研究  

The association between sno RNA profiles and prognosis in patients with gastric cancer

作　　者：韩璐[1] 宋丰举[1] 黄育北[1] 郑红[1] 陈可欣[1] 

机构地区：[1]天津医科大学肿瘤医院,国家肿瘤临床医学研究中心,天津市“肿瘤防治”重点实验室,肿瘤分子流行病与生物统计研究室,300060

出　　处：《天津医药》2016年第5期543-547,共5页Tianjin Medical Journal

基　　金：2014教育部“创新团队发展计划”资助项目(IRT_14R40)

摘　　要：目的：分析胃癌组织中snoRNA表达与胃癌预后的关联。方法胃癌患者90例，搜集其临床资料并随访。应用基因芯片技术，检测患者癌组织中405种snoRNA的表达水平，以每个snoRNA表达的中位数为界将胃癌患者分为高表达组和低表达组，进行单因素和多因素生存分析，比较2组患者总生存和无进展生存的差异。对存在差异的snoRNA按保护因素=0、危险因素=1拟合出危险评分。以年龄、性别、是否吸烟、是否饮酒、组织学分化（中高分化与低分化）、肿瘤大小（〈5 cm组和≥5 cm组）、肿瘤位置（上1/3与中下2/3）、临床分期（Ⅰ~Ⅱ期组和Ⅲ~Ⅳ期组）和snoRNA危险评分（低、中和高危组）为自变量，总生存和无进展生存为因变量，进行多因素Cox回归分析胃癌患者总生存和无进展生存的影响因素。结果19个snoRNA的高、低表达者间总生存和（或）无进展生存时间差异有统计学意义（P＜0.05）。Cox回归分析模型示，ACA61、ACA27和U36A高表达患者的总生存以及无进展生存较好，而ENSG00000206898高表达患者的总生存及无进展生存较差（P＜0.01）。snoRNA危险评分是胃癌患者总生存和无进展生存的独立影响因素；相较于低危组，中、高危组的总生存和无进展生存时间短（P〈0.001）。结论 ACA61、ACA27、U36A和ENSG00000206898的表达很可能是胃癌预后的独立预测因素，前3者低表达，后者高表达往往提示胃癌患者预后较差。Objective To identify snoRNA, which may be related to prognosis of gastric cancer. Methods Ninetygastric cancer patients who diagnosed at Tianjin Medical University Cancer Institute and Hospital were randomly collected in this study, and their clinical data were followed up. A total of 405 snoRNA expression profiles were analyzed in 90 gastric cancer patients. Patients were classified as＆quot;low expression＆quot;group or＆quot;high expression＆quot;group according to the median expression of each snoRNA expression, which was calculated by univariate and multivariate survival analysis. We also screened out the snoRNAs, in which patients were survived differently. Patients were classified as high, middle, or low risk groups based on the snoRNA risk score. Values of age, gender, smoking, drinking, histological differentiation （well, moderately-differentiated and poorly differentiated）, clinical stage （Ⅰ＋Ⅱstage andⅢ＋Ⅳstage）, tumor size （〈5 cm and≥5 cm）, tumor location （upper 1/3 and others） and snoRNA risk score （high, middle, and low risk group） were assessed by multivariate Cox analysis. Results There were significant differences in overall survival and （or） progression-free survival rates in 19 patients with high and low snoRNAs expressions （P〈0.05）. Results of multivariate Cox analysis showed that patients with high expression of ACA61,ACA27 and U36A showed a higher overall survival and progression-free survival rates, while patients with high expression of ENSG00000206898 showed a lower overall survival and progression-free survival survival rates （P〈0.01）. SnoRNA risk score is an independent prognostic factor for patients with gastric cancer. Compared with low risk group, patients in middle risk group and in high risk group showed a shorter overall survival and progression-free survival rates （P〈0.001）. Conclusion The expressions of ACA61, ACA27, U36A and ENSG00000206898 are independent prognostic factors of gastric cancer. Low expressions of the f

关 键 词：胃肿瘤 核仁小RNA 预后 基因表达谱 癌基因 因素分析 统计学 

分 类 号：R735.2[医药卫生—肿瘤]