鼠神经生长因子对全脑照射大鼠空间认知功能的修复作用  

Effect of mouse nerve growth factor on cognitive impairment in whole brain irradiation rats

作　　者：何国永[1] 黄海威[1] 邓哲治 郭俊杰[1] 

机构地区：[1]中山大学附属第一医院神经科,广州510080

出　　处：《中华医学杂志》2016年第19期1530-1534,共5页National Medical Journal of China

基　　金：国家自然科学基金（81271386）;广东省科技计划（20138021800286）

摘　　要：目的本实验通过观察x线照射前后大鼠神经功能的改变，使用鼠神经生长因子进行干预，探讨鼠神经生长因子（mNGF）对全脑照射大鼠的修复作用。方法10周龄雄性SD大鼠利用随机数字表法分为正常对照组（n=15）和照射组（n=40），正常对照组不作处理；照射组x线全脑照射12Gy剂量，照射30d后用随机数字表法分为mNGF组和生理盐水组。mNGF组肌注mNGF，生理盐水组照射等量生理盐水。三组大鼠照射60d后水迷宫实验检测空间记忆功能后取材，采用伊文思蓝（EB）法检测血脑屏障通透性，免疫荧光检测海马neuN、vWF、ZO-1蛋白表达，Western印迹检测大鼠脑部neuN、vWF、ZO．1、VEGF、GFAP的表达情况。结果水迷宫逃避潜伏期时间为正常对照组〈mNGF组〈生理盐水组，其跨越平台次数分别为（3．00±1．08）、（1．50±1．08）、（0．38±0．48）次，差异有统计学意义（P〈0．01）；依文思蓝染料渗出值分别为正常对照组（0．14±0．14）、mNGF组（0．66±0．20）、生理盐水组（1．36±0．27）μg／g，差异有统计学意义（P〈0．05）；免疫荧光中，海马区neuN、vWF、ZO-1蛋白阳性表达数量大小依次为正常对照组、mNGF组和生理盐水组；Western印迹中，正常对照组、mNGF组和生理盐水组3组的neuN、vWF、ZO-1蛋白表达依次降低，而GFAP和VEGF蛋白表达依次升高。结论鼠神经生长因子修复微血管、血脑屏障，促进神经元存活，两方面修复空间认知功能。Objective To investigate the positive effects of mouse nerve growth factor （mNGF） on cognitive impairment in whole brain irradiation rats. Methods Fifty-five male Sprague-Dawley rats were randomly divided into normal control group, mNGF treated group, saline treated group, mNGF treated group and saline treated group were cranial irradiated at a single dose of 12 Gy by X-ray. 30 days after radiation each group were treated with correspondent drugs. 60 days after radiation, Morris water maze experiment, EB leakage of the brain, and expressions of neuN, vWF, ZO-1 in hippoeampus by immunofluorescenee, and expressions of neuN, vWF, ZO-1, VEGF and GFAP in hippoeampus by Western blot were tested and analyzed. Results The escape latencies：normal control group 〈 mNGF treated group 〈 saline treated group ; the numbers of crossing hidden platform in these 3 groups were 3.00 ± 1.08, 1.50 ± 1.08, 0. 38 ± 0.48 times （P 〈0.01）. EB leakage of these 3 groups were 0.14 ±0.14, 0.66 ±0.20 and 1.36 ±0. 27μg/g （ P 〈 0.05 ） . In immunofluoreseenee, expressions of neuN, vWF and ZO-1 ： normal control group 〉 mNGF treated group 〉 saline treated group. In Western blot, expressions of neuN, vWF and ZO-1 ： normal control group 〉 mNGF treated group 〉 saline treated group, yet the expressions of VEGF and GFAP： normal control group 〈 mNGF treated group 〈 saline treated group. Conclusion mNGF ameliorates cognitive impairment after whole brain irradiation.

关 键 词：颅脑照射 神经生长因子 认知障碍 

分 类 号：R749.1[医药卫生—神经病学与精神病学]