IGF-I与IGF-IR在结肠癌中的表达及临床意义  

Expressions of IGF-I and IGF-IR in colon cancer and clinical significance

作　　者：陈吉添[1] 陈瑜[1] 吴薇紫[1] 陈虹[1] 沈晓梅[1] 

机构地区：[1]广西灵山县人民医院病理科,广西灵山535400

出　　处：《中国临床研究》2016年第5期581-585,589,共6页Chinese Journal of Clinical Research

基　　金：广西壮族自治区卫生厅科研课题(Z2011052)

摘　　要：目的探讨胰岛素样生长因子I(Insulin-like growth factor I,IGF-I)和IGF-I受体(IGF-IR)在结肠癌中的表达及其与临床病理因素和预后的相关性。方法利用逆转录聚合酶链反应(RT-PCR)方法对收集的114例结肠癌组织、136例结肠腺瘤组织和58例正常结肠组织中IGF-I及IGF-IR mRNA的表达情况进行分析,免疫组织化学法检测其蛋白表达情况。分析IGF-I及IGF-IR的表达与临床病理因素的关系。结果结肠癌组织中的IGF-I和IGF-IR的mRNA表达均高于结肠腺瘤组织、正常结肠组织,差异均有统计学意义(P均<0.05)。单因素分析显示,IGF-I和IGF-IR mRNA表达情况与结肠癌的分化程度、浸润深度及Dukes分期明显相关,差异有统计学意义(P均<0.05)。结肠癌组织IGF-IR蛋白的阳性表达率(70.2%)显著高于正常结肠组织(10.3%)(P<0.01)及结肠腺瘤组织(44.9%)(P<0.05)。结肠癌组织IGF-I蛋白的阳性表达率(62.3%)显著高于正常结肠组织(5.2%)(P<0.01)与结肠腺瘤组织(41.9%)(P<0.05)。单因素分析显示,IGF-IR的蛋白表达与Dukes分期、肿瘤分化程度、肿瘤大体类型有关(P<0.05,P<0.01);IGF-I的蛋白表达与Dukes分期、肿瘤分化程度有关(P<0.05,P<0.01)。多因素Cox回归分析结果显示,癌组织分化程度、Dukes分期、IGF-I和IGF-IR蛋白表达是影响结肠癌患者预后的独立因素(P<0.05,P<0.01)。结论 IGF-I及IGF-IR参与结肠癌的发生发展,同时可以作为预后因子,有助于判断结肠癌生物学行为和评价预后。Objective To investigate the expressions of insulin-like growth factor I（ IGF-I） and its receptor insulin-like growth factor I receptor（ IGF-IR） in colon cancer and the association of IGF-I and IGF-IR expressions with clinicopathological characteristics and prognosis. Methods Reverse transcriptase polymerase chain reaction（ RT-PCR） was used to analyze the expressions of IGF-I mRNA and IGF-IR mRNA in colon cancer tissues（ 114 cases）,colon adenoma tissues（ 136 cases） and normal colon tissues（ 58 cases）. Immunohistochemical method was used to detect the expressions of IGFI and IGF-IR proteins. The relationship between expressions of IGF-I and IGF-IR and clinicopathological characteristics was analyzed. Results The mRNA expression levels of the IGF-I and IGF-IR in colon cancer tissues was significantly higher than those in colon adenomas tissues and normal colon tissues（ all P 0. 05）. Single factor analysis showed that the mRNA expression levels of IGF-I and IGF-IR in colon cancer tissues were significantly associated with differentiation degree,invasion depth and Dukes staging of colon cancer（ all P 0. 05）. The positive expression rate of IGF-IR protein in colon cancer（ 62. 3%） was significantly higher than those in normal colon tissues（ 5. 2%）（ P 0. 01） and colon adenoma tissues（ 44. 9%）（ P 0. 05）. The positive expression rate of IGF-I protein in colon cancer（ 70. 2%） was significantly higher than those in normal colon tissues（ 10. 3%）（ P 0. 01） and colon adenoma tissues（ 41. 9%）（ P 0. 05）. Single factor analysis showed that the expression of IGF-IR protein was significantly associated with Dukes staging,differentiation degree,gross type of tumor,and the expression of IGF-I protein was significantly associated with Dukes staging and differentiation degree of tumor（ all P 0. 05）. Multifactor Cox regression analysis showed that the differentiation degree of cancer tissues,Dukes staging and the protein expressions of IGF-I and IGF-IR were the

关 键 词：胰岛素样生长因子I 胰岛素样生长因子I受体 结肠癌 淋巴结转移 浸润深度 DUKES分期 预后 

分 类 号：R735.3[医药卫生—肿瘤]