Hsa-miR-93-5p在喉鳞状细胞癌中的表达及其临床意义  

Expression of Hsa-miR-93-5p in laryngeal squamous cell carcinoma and its clinical significance

作　　者：张春明[1] 高伟[1] 王娜[1] 刘瑛[1] 赵沁丽[1] 李莹[1] 温树信[1] 王斌全[1] 

机构地区：[1]山西医科大学第一医院耳鼻咽喉-头颈外科耳鼻咽喉头颈肿瘤山西省重点实验室山西医科大学耳鼻咽喉研究所,太原030001

出　　处：《临床耳鼻咽喉头颈外科杂志》2016年第10期774-779,共6页Journal of Clinical Otorhinolaryngology Head And Neck Surgery

基　　金：国家自然科学基金资助项目(No:81172584);山西省青年科技研究基金(No:2015021198)

摘　　要：目的:探讨Hsa-miR-93-5p在喉鳞状细胞癌组织中的表达及对人喉鳞状细胞癌细胞株Hep-2细胞恶性表型的影响。方法:采用qRT-PCR检测喉鳞状细胞癌及配对癌旁正常黏膜石蜡包埋组织中Hsa-miR-93-5p的表达,并分析其与临床病理参数之间的关系;利用体外瞬时转染Hsa-miR-93-5p inhibitor化学合成物,采用MTS、Edu法和平板克隆形成实验检测转染后细胞活力、增殖及克隆形成能力的变化,采用Transwell小室检测细胞迁移和侵袭能力变化,采用流式细胞术检测细胞周期及凋亡的变化。结果:Hsa-miR-93-5p在喉鳞状细胞癌中的相对表达量为11.148±1.141,高于癌旁正常黏膜组织相对表达量为1.985±4.547(P<0.01)。在中低分化、T3+T4期以及颈淋巴转移(N+)喉鳞状细胞癌中,Hsa-miR-93-5p高表达例数构成比为69.8%、76.5%及89.5%,分别高于其在高分化、T1+T2及未发生颈淋巴转移(N0)中的构成比(P<0.05)。体外抑制Hep-2细胞中Hsa-miR-93-5p表达,可明显抑制细胞活力、增殖能力、平板克隆能力、迁移及侵袭转移能力,同时使细胞发生G2/M期阻滞,并促进其凋亡。结论:Hsa-miR-93-5p可能是喉鳞状细胞癌发生及进展过程中重要的癌基因,体外抑制其表达可抑制喉鳞状细胞癌细胞恶性表型,有望成为喉鳞状细胞癌分子靶向治疗的靶点之一。Objective：To investigate the expression of hsa-miR-93-5p on human laryngeal squamous cell carcinoma and the influence on malignant phenotype of Hep-2cell.Method：The expression of hsa-miR-93-5p of paraffin samples in LSCC was determined by looped-primer Real-time PCR,and the relationship between the expression and the clinical pathological parameters was analysed.The hsa-miR-93-5p Inhibitor sequence was transfected into Hep-2cells as the Inhibitor group.Using the MTS assay,Edu and colony formation assay to investigate the change of cell viability,proliferation and clone formation ability after transfection.Transwell invasion assay was used to detect the changes of cell migration and invasion ability.Flow cytometry was used to detect the changes of cell cycle and apoptosis.Result：The relative expression of hsa-miR-93-5p in LSCC was 11.148±1.141,which was higher than in normal tissues of adjacent to carcinoma1（985±4.547）（P〈0.01）.The constituent ratio of hsamiR-93-5p high expression in the group of low differentiation,T3＋T4and lymphatic metastasis was 69.8%、76.5% and 89.5%,which was higher than the group of high differentiation,T1＋T2and non-lymphatic metastasis respectively（P〈0.05）,Inhibition the expression of hsa-miR-93-5p in vitro in Hep-2cells could obviously inhibit the cell vitality,proliferation,clone,migration,and invasion ability,also could retardant the cells in G2/M phase,and promote its apoptosis.Conclusion：Hsa-miR-93-5p might be an important molecule in pathogenesis of laryngeal squamous cell carcinoma.It could inhibit malignant phenotype of laryngeal squamous cancer cells whenhsamiR-93-5p expressionwas suppressed in vitro.

关 键 词：喉肿瘤 鳞状细胞癌 hsa-miR-93-5p 增殖 侵袭转移 

分 类 号：R739.6[医药卫生—肿瘤]