釉原蛋白对人牙周膜干细胞迁移、粘附及增殖能力的影响  

Effect of amelogenin on the migration,adhesion and proliferation of periodontal ligament stem cells

作　　者：赵希[1] 韩玉嘉[2] 熊宇[1] 

机构地区：[1]第三军医大学西南医院口腔科,重庆400038 [2]重庆市沙坪坝区人民医院口腔科,重庆400030

出　　处：《局解手术学杂志》2016年第5期327-330,共4页Journal of Regional Anatomy and Operative Surgery

基　　金：重庆市卫生计生委科研项目(2015XMSB0001369)

摘　　要：目的探究釉原蛋白(AML)对牙周膜干细胞(PDLSCs)迁移、粘附和增殖的影响。方法流式细胞仪分选Stro-1+PDLSCs,采用Transwell法检测AML对PDLSCs迁移的影响,粘附实验检测AML对PDLSCs粘附的影响,MTT检测AML对PDLSCs增殖能力的影响。结果 Transwell结果显示,AML能够作用于PDLSCs,促进其迁移,并且此影响呈正相关;粘附实验结果显示,PDLSCs的粘附同样受AML的影响,该作用随培养时间延长而增加;MTT结果显示,AML能够促进PDLSCs增殖,且随着剂量增加该促进作用增强。结论体外AML能够作用于PDLSCs,促进其迁移、粘附和增殖。Objective To study the effect of amelogenin（ AML） on the migration,adhesion and proliferation of periodontal ligament stem cells（ PDLSCs）. Methods STRO-1 ＋ PDLSCs were selected by Flow cytometer. The transwell system was used to detect the effect of AML on migration of PDLSCs. Cells attachment assay was used to detect the effect of AML on adhesion of PDLSCs. MTT was used to detect the effect of AML on the proliferation of PDLSCs. Results The results of transwell showed that AML could induce the migration of PDLSCs with positive correlation. Cells attachment assay showed that the adhesion of PDLSCs was effected by AML as well,and the effect was increased with the extension of incubation time. Results of MTT showed that AML could promote the proliferation of PDLSCs in a dose dependent manner. Conclusion AML could increase the proliferation,adhesion and migration potential of PDLSCs.

关 键 词：牙周膜干细胞 釉原蛋白 迁移 增殖 粘附 

分 类 号：R781.42[医药卫生—口腔医学]