微小RNA-103靶向调控小窝蛋白1改善蛛网膜下腔出血后神经功能缺损  

Target-regulated caveolin-1 by miR-103 improves neurological deficits following subarachnoid hemorrhage

作　　者：王刘敏 谢怡[1] 许丽丽[1] 叶瑞东[1] 刘新峰[1] 

机构地区：[1]南京大学医学院附属金陵医院南京军区南京总医院神经内科,210002

出　　处：《中华老年心脑血管病杂志》2016年第5期531-534,共4页Chinese Journal of Geriatric Heart,Brain and Vessel Diseases

基　　金：国家自然科学基金(81200892;31300900;81471182;81400332)

摘　　要：目的探讨微小RNA-103(miR-103-3p)在蛛网膜下腔出血(SAH)后的表达情况以及对SAH后神经功能的影响,并探究其分子机制。方法将60只雄性SD大鼠随机分为3组,假手术组12只,对照组24只和实验组24只。后2组采用血管内刺破法制造大鼠SAH模型前分别注射miR-103-3p空白对照和抑制剂。利用实时荧光定量PCR技术比较各组miR-103-3p mRNA的表达情况,Western blot技术检测各组小窝蛋白1(Cav-1)蛋白的表达情况,采用改良Garcia评分及平衡木实验评估各组SAH术后24h及7d神经功能改变。结果与假手术组比较,对照组miR-103-3p在SAH后24h表达上调3.61倍,Cav-1表达下调46%。与对照组比较,实验组miR-103-3p在SAH术后24h表达下调63%,Cav-1表达上调2.47倍。实验组大鼠SAH术后24h[(11.9±2.6)分vs(9.2±3.2)分]及7d[(12.4±2.6)分vs(9.5±2.4)分]较对照组神经功能缺损评分明显升高(P<0.05)。结论miR-103-3p在SAH术后24h表达明显升高,抑制miR-103-3p可通过增加Cav-1的表达,从而有效改善大鼠SAH术后神经功能缺损。Objective To study the expression of miR-103-3p on neurological deficits following subarachnoid hemorrhage（SAH）and its mechanism.Methods Sixty male SD rats were divided into sham operation group（n=12）,control group（n=24）and experimental group（n=24）.A SAH model was established by endovascular perforation.Expression of miR-103-3p and caveolin-1was detected by quantitative PCR and Western blot respectively.The neurological function of rats was assessed on days 1and 7after SAH according to the modified Garcia Scoring System and beam balance test.Results The miR-103-3p expression level was significantly higher whereas the caveolin-1expression level was significantly lower in control group than in sham operation group at 24 hafter SAH.The miR-103-3p expression level was significantly lower whereas the caveolin-1expression level was significantly higher in experimental group than in control group at 24 hafter SAH.The neurological function score was significantly higher in experimental group than in control group on days 1and 7after SAH（11.9±2.6 vs 9.2±3.2,12.4±2.6 vs 9.5±2.4,P〈0.05）.Conclusion The miR-103-3p expression level is significantly higher in rats at 24 hafter SAH.The neurological function of rats can be effectively improved by down-regulating the expression of miR-103-3p and up-regulating the expression of caveolin-1.

关 键 词：微RNAS 蛛网膜下腔出血 窖蛋白1 氧化性应激 细胞凋亡 

分 类 号：R743.35[医药卫生—神经病学与精神病学]