两种介体物质在漆酶降解磺胺类抗生素中的作用  

Role of two mediators in sulfonamide antibiotics degradation by laccase oxidation system

作　　者：丁惠君[1,2,3] 吴亦潇[1] 钟家有[2,3] 邹斌春 张维昊[1] 楼倩[2,3] 

机构地区：[1]武汉大学资源与环境科学学院,湖北武汉430072 [2]江西省水利科学研究院,江西南昌330029 [3]江西省鄱阳湖水资源与环境重点实验室,江西南昌330029

出　　处：《中国环境科学》2016年第5期1469-1475,共7页China Environmental Science

基　　金：江西省水利厅重大科技项目(KT201412);水利部鄱阳湖水资源水生态环境研究中心基金项目(kfjj201403);国家重点基金项目(41331174);湖北省环境保护厅环保科研项目(2014HB08)

摘　　要：通过比较两种介体物质丁香醛（SA）和1-羟基苯并三氮唑（HBT）对漆酶降解5种典型磺胺类抗生素的影响,考察了介体的起始浓度、p H值、反应温度、漆酶起始浓度等因子的影响,得到介体SA对漆酶催化降解磺胺类抗生素的效果更好.在漆酶＋SA反应体系中,反应体系p H值为5～6,反应温度为30℃,SA起始浓度为0.5mmol/L,漆酶起始浓度为0.5mg/m L时,漆酶对5种磺胺类抗生素的降解效果最佳,降解去除率在15min时达到75%左右,1h内均达到97%以上.本研究表明,适当的介体物质可大大提升漆酶对磺胺类抗生素的降解效果.漆酶在介体物质的作用下对抗生素类污染物的生态净化具有良好的应用潜力.Two kinds of mediators,namely syringaldehyde（SA） and1-hydroxybenzotriazole（HBT）,were investigated in laccase oxidation system,regarding the degradation of five kinds of typical sulfonamide antibiotics.The influences of factors,such as the initial concentrations of mediators,p H,temperature and the concentration of laccase,were studied.The results showed that SA was more effective in mediating laccase oxidation of all five kinds of sulfonamide antibiotics.In laccase＋SA reaction system,the optimum conditions for the degrading the sulfonamide antibiotics by laccase were：at p H5~6 and 30℃,and with initial concentrations of SA and laccase respectively 0.5mmol/L and 0.5mg/m L.The degradation rates reached around 75% in 15 minutes and more than 97% within 1hour for all five kinds of sulfonamide antibiotics.This study showed that the appropriate mediator can greatly improve laccase oxidation.Laccase oxidation assisted by mediators has a good application potential for the ecological purification of antibiotic pollutants.

关 键 词：丁香醛 1-羟基苯并三氮唑 漆酶 磺胺类抗生素 降解 

分 类 号：X171[环境科学与工程—环境科学] X131