毛细管区带电泳化学发光法测定食品中残留的磺胺类药物  

Determination of Sulfonamides Residues in Food by Capillary Zone Electrophoresis-Chemiluminescence Method

作　　者：代婷婷[1] 林华萍[1] 段婕[1] 徐向东[1] 石红梅[1] 

机构地区：[1]河北医科大学公共卫生学院,石家庄050017

出　　处：《分析化学》2016年第5期747-753,共7页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金项目(Nos.81273128,81402723)资助

摘　　要：基于碱性介质中,磺胺类药物对Ag？配合物与鲁米诺（Luminol,Lu）化学发光体系的发光强度有抑制作用,建立了毛细管电泳-化学发光分离检测磺胺甲噁唑（Sulfamethoxazole,SMZ）、磺胺二甲氧嘧啶（Sulfadimethoxine,SDM）、磺胺噻唑（Sulfathiazole,ST）的方法。3种磺胺类药物经毛细管电泳分离后,分别与Ag？配合物和鲁米诺化学发光体系作用,以相对迁移时间定性,相对化学发光强度定量,采用标准曲线法测定样品中的待测物含量。对影响毛细管电泳的分离与化学发光检测的条件均进行了优化。在最佳分离检测条件下,3种磺胺类药物在2.0~200μg/m L范围内线性良好（r〉0.9977）。对3种磺胺类药物平行测定7次,相对标准偏差（RSD）为1.3%~1.9%,3种磺胺类物质的检出限分别为0.33,0.20和0.034μg/m L,加标回收率为80.2%~102.9%。将本方法应用于猪肉、鸡肉、牛奶中的3种磺胺类药物残留量检测,结果令人满意。Based on sulfonamides inhibited the chemiluminescence （CL） intensities of the system consisting of Ag（~ complexes with Luminol （Lu） in basic medium, a method for capillary eleetrophoresis （CE）-CL separation and determination of sulfamethoxazole （SMZ）, sulfadimethoxine （SDM） and sulfathiazole （ST） was established. The three kinds of sulfonamides were separated by capillary electrophoresis, and reacted with Ag（Ⅲ） complexes-Luminol CL system, respectively. In the relative migration time, the relative CL intensity was quantitative and the standard curve method was used to determine the content of the analytes in the sample. The conditions for the separation and chemiluminescence detection system were all optimized. Under the optimized conditions, the linear ranges for the determination of SMZ, SDM and ST were 2.0-200.0 μg/mL （ r〉0. 9977 ）, with detection limits （ S/N= 3 ） of 0.33, 0.20 and 0. 034 μg/mL, respectively. Relative standard deviation （RSD） values for the peak height were 1.3% to 1.9% （n = 7）. The proposed method was applied to the determinations of sulfonamides residues in pork, chicken, milk samples with satisfactory results.

关 键 词：毛细管电泳 化学发光 Ag(Ⅲ)配合物 磺胺类药物 

分 类 号：TS207.5[轻工技术与工程—食品科学] O657.3[轻工技术与工程—食品科学与工程]