不同n-6/n-3脂肪酸构成对小鼠心血管疾病危险因素的影响  

Effects of diets with different n-6/n-3 fatty acids on cardiovascular risk factors in mice fed high-fat diets

作　　者：杨立刚[1] 宋志秀[2] 曹卫鑫 王炎炎[1] 芦慧霞[3] 郭斐[3] 杨辉军[1] 陈剑双[1] 王少康[1] 孙桂菊[1] 

机构地区：[1]东南大学公共卫生学院环境医学工程教育部重点实验室/营养与食品卫生学系,南京210009 [2]南京中医药大学第二临床医学院 [3]东南大学附属中大医院

出　　处：《卫生研究》2016年第3期436-441,共6页Journal of Hygiene Research

基　　金：国家自然科学基金(No.81001244);江苏省高等学校大学生实践创新训练项目(No.S2015137;S2014115)

摘　　要：目的研究不同脂肪酸构成对小鼠血脂代谢、炎症和氧化应激及内皮细胞功能的影响。方法雄性KM小鼠随机分为5组,分别喂饲正常对照饲料、猪油高脂饲料和n-6/n-3多不饱和脂肪酸(PUFA)比值为1∶1、5∶1、20∶1的高脂饲料5周,比较各组小鼠血清中甘油三酯(TG)、总胆固醇(TC)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、白介素-6(IL-6)、丙二醛(MDA)、超敏C反应蛋白(hsCRP)、肿瘤坏死因子(TNF-α)、脂质过氧化物(LPO)、8-异前列腺素F2α(8-isoPGF2α)、氧化型低密度脂蛋白(ox-LDL)、游离脂肪酸(FFA)、选择素(ES)和血管性血友病因子(v WF)的含量变化。结果猪油组LDL-C和非高密度脂蛋白胆固醇(non-HDL-C)水平显著高于其他各组(P<0.05)。n-6/n-3 PUFA 1∶1组和5∶1组血清TG、TC水平显著低于猪油组(P<0.05)。20∶1组血清FFA水平显著高于1∶1组和5∶1组(P<0.05)。1∶1组和5∶1组血清炎症因子和氧化应激指标及ES水平均显著低于猪油组和20∶1组(P<0.05)。5∶1组血清v WF水平显著低于猪油组和20∶1组(P<0.05)。结论与猪油和高n-6/n-3 PUFA比值高脂饲料相比较,低n-6/n-3PUFA比值高脂饲料可改善小鼠脂代谢、炎症与氧化应激和内皮细胞功能。Objective To investigate effects of diets with different fatty acid composition on serum lipid profiles, inflammation, oxidative stress and endothelial function in mice fed high-fat diets. Methods Male KM mice were randomly divided into five groups and were fed normal control diet,high-fat lard diet,high-fat diets with n-6 / n-3 polyunsaturated fatty acid（ PUFA） ratios of 1 ∶ 1,5 ∶ 1 and 20 ∶ 1 for five weeks,respectively. The levels of serum triglyceride（ TG）,total cholesterol（ TC）,high-densitylipoprotein cholesterol（ HDL-C）, low-density lipoprotein cholesterol（ LDL-C）,interleukin-6（ IL-6）,malondialdehyde（ MDA）,hypersensitive C-reactive protein（ hsCRP）,tumor necrosis factor alpha（ TNF-α）,lipid peroxide（ LPO）,8-iso prostaglandin F2α（ 8-iso-PGF2α）,oxidized low-density lipoprotein（ ox-LDL）,free fatty acid（ FFA）,E-selectin（ ES） and von Willebrand factor（ v WF） were measured. Results The levels of serum LDL-C and non-HDL-C in the lard group were significantly higher than those in the other groups（ P 0. 05）. The lard group had significantly higher serum TG and TC concentrations compared to 1∶ 1 and 5∶ 1 groups（ P〈0. 05）. The levels of serum FFA in20∶ 1 group were significantly higher than those in 1 ∶ 1 and 5 ∶ 1 groups（ P〈0. 05）.Compared with the lard and 20∶ 1 groups,the 1∶ 1 and 5∶ 1 groups displayed lower levels of inflammatory cytokines,oxidative stress and ES. The 5∶ 1 group significantly decreased the level of serum v WF compared to lard and 20 ∶ 1 groups（ P〈0. 05）. Conclusion High-fat diet with low n-6 / n-3 PUFA ratio could improve lipid metabolism,inflammation,oxidative stress and endothelial function compared to high-fat diets with lard and higher n-6 / n-3 PUFA ratio. The diet with low n-6 / n-3 PUFA ratio can improve cardiovascular disease risk factors to prevent cardiovascular disease.

关 键 词：脂肪酸 n-6/n-3多不饱和脂肪酸比值 脂代谢 氧化应激 内皮细胞功能 

分 类 号：R54[医药卫生—心血管疾病] Q591.5[医药卫生—内科学]