酚酸类物质对铜绿微囊藻以及蛋白核小球藻的抑藻作用  

Joint inhibitory effects researches on Microcystis aeruginosa and Chlorella pyrenoidosa of phenolic acids

作　　者：张庭廷[1] 韩玉珍[1] 何宗祥[1] 汪好芬[1] 

机构地区：[1]安徽师范大学生命科学学院,生物环境与生态安全安徽省高校重点实验室,安徽省皖江城市带退化生态系统的恢复与重建协同创新中心,芜湖241000

出　　处：《卫生研究》2016年第3期448-451,457,共5页Journal of Hygiene Research

基　　金：国家自然科学基金(No.31170443)

摘　　要：目的研究单一以及复合酚酸对混合藻类的抑制作用。方法选用水杨酸、肉桂酸与焦性没食子酸单一或两两结合作用于铜绿微囊藻(Microcystis aeruginosa)与蛋白核小球藻(Chlorella pyrenoidosa)的复合体系,以叶绿素为生物量指标,计算其单一酚酸抑藻的半最大效应浓度(EC_(50)),判断其联合抑藻的复合效应。结果三种酚酸单独对铜绿微囊藻和蛋白核小球藻混合藻类均有一定抑制作用,抑制作用为焦性没食子酸>水杨酸>肉桂酸,实验第6 d,这三种酚酸单独对铜绿微囊藻和蛋白核小球藻混合体系抑制作用的EC_(50)依次为:7.87、64.90以及89.34 mg/L;复合酚酸对混合藻的抑制作用为焦性没食子酸与水杨酸组合>焦性没食子酸与肉桂酸组合>水杨酸与肉桂酸组合,前两种组合表现为协同抑藻作用,后一种组合的抑藻效应为相加作用。结论某些酚酸联合应用可协同抑藻,从而能减少其单一酚酸的使用量,具有相对较高的环境安全性。Objective To phenolic acids on mixed algae. acid were chosen individually or Chlorella pyrenoidosa. Results inhibitory effects on the mixed investigate the inhibitory effects of single and compound Methods Salicylic acid, cinnamic acid and pyrogallic in pairs to act on mixed algae of Microcystis aeruginosa and Three phenolic acids that singled or paired showed certain algae, of which were pyrogallic acid 〉 salicylic acid 〉 cinnamic acid in single phenolic acid, the ECs08of the three phenolic acids on the mixture of Microcystis aeruginosa and Chlorella pyrenoidosa were 7.87, 64.90 and 89.34 mg/L at the day 6 ; and inhibition of the paired phenolic acid group was pyrogallic acid ＆ salicylic acid group 〉 pyrogallic acid ＆ cinnamic acid group 〉 salicylic acid ＆ cinnamic acid, the first 2 groups had synergistic algal inhibitory effects and the last paired group showed additive action. Conclusion Some specifically combined phenolic acids can lead to synergistic inhibiting effects on algae, which has better environmental safety for application due to the use of single phenolic acid was massively decreased.

关 键 词：酚酸 铜绿微囊藻 蛋白核小球藻 协同抑藻 EC50 

分 类 号：X17[环境科学与工程—环境科学] X524