橘核角鲨烯合成酶基因(ss)的克隆、分析及表达  

Cloning,Analysis and Expression on Gene of Squalene Synthase in Citrus reticulata Seeds

作　　者：吴沂芸 罗静[1] 王黎[1] 王倩[1] 裴瑾[1] 

机构地区：[1]成都中医药大学药学院,四川成都611137

出　　处：《中药材》2016年第2期270-274,共5页Journal of Chinese Medicinal Materials

基　　金：国家自然科学基金人才项目(J131003340-13)

摘　　要：目的:从大红袍Citrus reticulata‘Dahongpao’种子的总RNA中克隆角鲨烯合成酶基因(ss),并对其进行生物信息学及表达分析。方法:采用RT-PCR技术克隆基因并对其在橘核不同生长时期的表达进行分析,利用在线分析工具对基因进行生物信息学分析。结果:克隆所得ss总长度为1 205 bp,编码397个氨基酸,与芸香科柑橘属Citrus L.甜橙Citrus sinensis、克莱檬蒂娜橘Citrus clementina角鲨烯合成酶(SS)蛋白质序列具有99%的相似性。将橘核生长发育过程分为形态建成末期、成熟期和脱水期3个阶段,其中,种子成熟期的ss表达量最高。结论:首次从大红袍中成功克隆ss,可为橘核中柠檬苦素类化合物生物合成及调控机制的研究提供参考。Objective：To clone squalene synthase gene（ss）from seeds of Citrus reticulata ＇ Dahongpao＇ , and to analyze its expres- sion and bioinformatics. Methods：The technology of RT-PCR was used to clone the gene of Citrus reticulata seeds and analyze its ex- pression at various growing stages. Moreover, the bioinformatics was analyzed by online analytical tools. Results ： The full-length of ss was 1 205 bp and 397 amino acids were encoded, SS had a homology of 99% with that of Citrus sinensis and Citrus clementina. The growth process of Citrus reticulata seeds was divided into three stages ：morphogenesis stage, maturity and dehydration periods, and expression of ss was the highest in maturity period. Conclusion ： Squalene synthase gene from seeds of Citrus retieulata ＇ Dahongpa＇ is obtained for the first time, which provides reference for biosynthesis and regulation mechanism research of limonin compounds in Citrus retieulata seeds.

关 键 词：大红袍 橘核 角鲨烯合成酶 克隆 生物信息学分析 基因表达 

分 类 号：R282.71[医药卫生—中药学]