逍遥丸药渣的纤维素酶解工艺优化  

Optimization of Enzymatic Hydrolysis Conditions of Xiaoyao-pill Herb Residues by Cellulase

作　　者：任海伟[1,2] 裴芳霞 张丙云[1,2] 王永刚[1,2] 蒲秀瑛[1,2] 张轶[1,2] 王莲萍[2] 

机构地区：[1]甘肃省生命科学与食品工程实验教学示范中心/甘肃省高校中藏药筛选评价及深加工重点实验室,甘肃兰州730050 [2]兰州理工大学生命科学与工程学院,甘肃兰州730050

出　　处：《中药材》2016年第2期358-362,共5页Journal of Chinese Medicinal Materials

基　　金：国家自然科学基金(51366009);甘肃省高等学校科研项目(2014A-030);甘肃省自然科学基金(145RJZA064;148RJZA015);兰州市科技计划项目(2014-2-20);兰州理工大学红柳青年教师培养计划(Q201207)

摘　　要：目的:探讨逍遥丸药渣的纤维素酶解工艺参数。方法:在单因素试验基础上,选取纤维素酶添加量、酶解时间、料液比为影响因素,酶解得率为响应值,运用Box-Behnken设计对逍遥丸药渣的纤维素酶解工艺参数进行优化,建立数学回归模型。结果:逍遥丸药渣的纤维素酶解优化工艺参数为:纤维素酶添加量6%、酶解时间5.6 h、料液比1∶12(g/m L),在该条件下的酶解得率高达43.89%。结论:该方法能够获得较高的发酵糖,为中药渣的资源化利用奠定基础。Objective: To explore the enzymatic hydrolysis conditions of Xiaoyao-pill herb residues by cellulose. Methods: Based on the single factor test,an Box-Benhnken design was used to optimize the parameters,three factors,including cellulase dosage,enzymolysis time,and solid-liquid ratio were regarded as investigation factors,the yield of enzymatic hydrolysis as index,Xiaoyao-pill herb residues enzymatic hydrolysis catalyzed by cellulase,and a mathematical regression model was established. Results: The optimal parameters were obtained as follows,cellulase dosage was 6%,enzymolysis time was 5. 6 h and solid-liquid ratio was 1 ∶ 12( g / m L). Under this condition,the yield of enzymatic hydrolysis was 43. 89%. Conclusion: The results may provide new reference for further exploring Chinese herbal medicine efficiently.

关 键 词：逍遥丸 药渣 酶解糖化 纤维素酶 

分 类 号：R286.3[医药卫生—中药学]