MTA1基因表达载体的构建与鉴定  

Construction and identification of the eukaryotic express vector of MTA1 gene

作　　者：黎桂仙 陈洪浪[3] 姚运红[2] 胡新荣[1,2] 姜恩平[1,2] 朱伟[1,2] 

机构地区：[1]广东医学院肿瘤研究所,广东东莞523808 [2]广东医学院病理学教研室,广东东莞523808 [3]广东医学院药学院,广东东莞523808

出　　处：《广东医学院学报》2015年第6期640-643,共4页Journal of Guangdong Medical College

基　　金：国家自然科学基金(No.81472275);广东省自然科学基金(No.2014A030313542);广东医学院博士启动基金(No.B2011004;B2012063)

摘　　要：目的构建转移相关基因1(metastasis-associated gene 1,MTAl)的真核表达载体。方法采用RT-PCR方法扩增出MTAl基因片段,通过DNA重组技术将基因片段连接于p IRES2-e GFP真核表达质粒载体上,采用质粒PCR、酶切电泳分析及DNA测序对质粒进行鉴定。结果质粒PCR及DNA测序鉴定结果均表明载体构建正确。结论成功构建了MTAl的真核表达载体p IRES2-MTAl,为深入研究MTAl基因在结直肠癌发生发展中的作用及机制提供前期实验基础。Objective To construct and identify the eukaryotic express vector of metastasis-associated gene 1（MTA1）. Methods MTA1 gene was obtained by reverse transcription-polymerase chain reaction（RT-PCR）,then transfected to eukaryon expression plasmid of p IRES2-e GFP. The plasmid was identified by PCR,double enzyme digestion analysis and DNA sequencing to evaluate the recombinant. Results The plasmid PCR and DNA sequencing results all showed that the vector was constructed correctly. Conclusion The MTA1 eukaryotic express vector was successfully constructed and named as pIRES2-MTA1,laying foundation for further research on the role and mechanism of MTA1 gene in colorectal cancer.

关 键 词：转移相关基因1 真核表达载体 结直肠癌 

分 类 号：R363[医药卫生—病理学]