慢性失神经后衰老相关β-半乳糖苷酶在成年大鼠施万细胞的表达  

Senescence-associated Beta Galactosidase Expression in Rat Schwann Cells after Chronic Denervation

作　　者：肖霞[1] 陈泽卡[1] 丁有权 李轩漾[1] 齐建国[1] 

机构地区：[1]四川大学华西基础医学与法医学院组织胚胎学与神经生物学教研室,成都610041

出　　处：《四川大学学报（医学版）》2016年第3期351-355,共5页Journal of Sichuan University(Medical Sciences)

基　　金：四川省科技厅科技支撑计划项目(No.2013SZ0069)资助

摘　　要：目的探讨成年大鼠长时程轴突缺失对大鼠施万细胞中衰老相关β-半乳糖苷酶(senescenceassociated beta galactosidase,SA-β-gal)表达的影响。方法成年雄性SD大鼠随机分为假手术组、1周失神经组、2周失神经组、4周失神经组、5周失神经组、6周失神经组、7周失神经组和8周失神经组。各组动物进行右侧坐骨神经永久性横断手术或者相应的假手术,根据各组设定的远侧段失神经时间分别获取损伤神经的5mm近侧段和10mm远侧段以及假手术组同侧神经和手术组对侧神经的对应节段,采用SA-β-gal染色检测SA-β-gal的表达,并对8周失神经组大鼠获取的损伤神经标本用SA-β-gal和施万细胞特异性蛋白(S100β)双标染色分析远侧段神经中SA-β-gal表达的细胞定位。结果成年大鼠右侧坐骨神经永久横断后,近侧段神经的远端SA-β-gal表达在2周一过性增加,而远侧段神经中SA-β-gal表达在2周明显增加,并且在更长的失神经过程中保持这种高表达水平。而且,绝大部分SA-β-gal的表达出现在S100β免疫反应阳性的施万细胞中。结论长时程轴突缺失增加成年大鼠施万细胞中SA-β-gal表达。Objective To investigate the effect of prolonged axon depletion on senescence-associated beta galactosidase（SA-β-gal）expression in Schwann cells（SCs）of adult rats.Methods Male adult Sprague-Dawley（SD）rats were randomize grouped into sham-operated group and denervation groups for 1week,2weeks,4weeks,5weeks,6weeks,7weeks and 8weeks.Rats were subjected to right sciatic nerve transection.After particular denervation duration for the distal stumps,animals were anesthetized and perfused.Proximal stumps of 5mm and distal stumps of 10 mm from injured nerves,and the corresponding segments from the sham groups and contralateral nerves were harvested and prepared for SA-β-gal staining to detect SA-β-gal expression.Then,additional injured distal stumps denervated for 8weeks were employed for determining cellular distribution of SA-β-gal expression by co-labeling of SA-β-gal and SC-specific protein（S100β）.Results SA-β-gal expression transiently increased in distal tips of proximal stumps 2weeks after adult rat sciatic nerve transection without suture.In contrast,in the distal stumps of transected adult rat sciatic nerves,axon depletion for 2 weeks increased SA-β-gal expression,and the increased expression of SA-β-gal remained constant after prolonged denervation durations. Furthermore,combination of SA-β-gal staining with S100βimmunofluorescence staining showed that SA-β-gal expression was exclusively present in denervated SCs.Conclusion Prolonged axon depletion increased SA-β-gal expression in adult rat SCs.

关 键 词：慢性失神经 施万细胞 细胞衰老 衰老相关β-半乳糖苷酶 坐骨神经横断 

分 类 号：R741[医药卫生—神经病学与精神病学]