镍铬合金修复体对龈沟液中LDH同工酶谱的影响  

INFLUENCE OF LACTATE DEHYDROGENASE ISOENZYME IN GINGIVAL CREVICULAR FLUID AFTER NI-CR ALLOY DENTAL RESTORATION

作　　者：周俊红 周立社[2] 秦文斌[2] 雎天林[2] 李利[3] 

机构地区：[1]北京市中关村医院口腔科,北京100190 [2]包头医学院生物化学教研室 [3]内蒙古医科大学第四附属医院口腔科

出　　处：《内蒙古医科大学学报》2016年第1期58-60,64,共4页Journal of Inner Mongolia Medical University

基　　金：北京市海淀区科学技术委员会科技发展计划资助项目

摘　　要：目的：探讨镍铬合金修复牙体后龈沟液（gingival crevicular fluid,GCF）中乳酸脱氢酶（lactate dehydrogenase,LDH）同工酶的变化。方法：选择镍铬合金修复1-2a的牙位58个,健康牙位54个,用滤纸条法采集GCF,醋酸纤维薄膜电泳结合光密度扫描法测定GCF-LDH同工酶的相对含量。结果：金属修复牙组与健康对照组比较,两组间GCF中LDH1、LDH2、LDH3和LDH5的相对含量均无显著性差异（t1、t2、t3、t5分别为0.49、1.56、0.12、0.37;P〉0.05）,但镍铬合金修复组GCF-LDH4相对含量明显高于健康组（t4=2.03,P4〈0.05）。结论：镍铬合金修复牙GCF中的LDH同工酶谱有变化,提示镍铬合金对牙周组织可能有刺激或损伤作用。Objective：To investigate the changes of lactate dehydrogenase （ LDH ） isoenzyme in gingival crevicular fluid（ GCF） after Ni-Cr alloy dental restoration. Methods：Fifty-eight prothetic teeth by Ni-Cr alloy and fifty-four healthy teeth were selected,GCF was sampled with filter paper strips,the relative content of GCF-LDH isoenzyme was detected using cellulose acetate membrance eletrophoresis and densitometric scaning. Result：There was no significant difference in the relative content of LDH1 , LDH2,LDH3 and LDH5 between prothetic teeth group and healthy teeth group（t1,t2,t3,t5 is 0.49, 1. 56,0. 12, 0. 37;all P〉0. 05 ）, but the GCF-LDH4 content in the prothetic teeth group was significantly higher than it in the healthy teeth group（t4=2. 03,P4〈0. 05）. Conclusion：The GCF-LDH isoenzyme-gram of Ni-Cr alloy prothetic teeth is changed. It indicates that Ni-Cr alloy dental prosthesis is likely to have a stimulation or injury to periodontium.

关 键 词：镍铬合金 修复体 乳酸脱氢酶 龈沟液 

分 类 号：R783[医药卫生—口腔医学]