氨基葡甘露聚糖对人呼吸道合胞病毒的体内抑制作用  

Inhibitory effect of aminoglucomannan on human respiratory syncytial virus in vivo

作　　者：苏秀峰[1] 李娜[2] 陈国民[3] 陈旭芳[1] 

机构地区：[1]滨州医学院烟台附属医院肿瘤一科,山东烟台264100 [2]滨州医学院烟台附属医院老年医学科,山东烟台264100 [3]重庆医科大学生物医学工程学院,400016

出　　处：《中华临床感染病杂志》2016年第2期173-179,共7页Chinese Journal of Clinical Infectious Diseases

基　　金：国家科技部新药研究基金（96-901-05-240）

摘　　要：目的：探讨氨基葡甘露聚糖（AGM）在小鼠体内抗人呼吸道合胞病毒（hRSV）的作用。方法建立hRSV感染的BALB／c小鼠模型108只，随机分为AGM组和魔芋葡甘露聚糖（ KGM）组，每组54只，分别以AGM和KGM进行干预，然后按单次给药剂量不同，将两组小鼠进一步分为2.5、0．25和0．025μmol／L 3组，每组再根据给药间隔时间（8、12和24 h）又分为3组，共9个小组，每组6只。72 h后处死小鼠，观察小鼠一般情况的变化；采用反转录聚合酶链反应（ RT-PCR）方法检测两组小鼠肺组织中hRSV融合蛋白（ F） mRNA的水平；对两组小鼠肺组织行HE染色和细胞间黏附分子-1（ICAM-1）的免疫组化检测，分析肺组织炎症状况。采用随机区组设计的方差分析检验组内给药剂量和给药间隔时间对实验结果的影响。结果 AGM组小鼠活动无明显减弱，无明显呼吸困难，无死亡；KGM组有16只小鼠活动明显减弱，出现明显喘息症状，其中2只于第3日死亡。 RT-PCR结果显示， AGM组和KGM组的 hRSV F mRNA 水平分别为0．49＆#177;0．21和0．88＆#177;0．06，差异有统计学意义（t＝6.71，P＜0．05）；小鼠肺组织HE染色显示，AGM组炎症比KGM组轻。免疫组化结果显示，AGM组小鼠肺组织中ICAM-1的表达量较KGM组明显减少，差异有统计学意义（ t＝13．88，P＜0．05）。AGM组内，给药间隔时间一定时，hRSV F mRNA和ICAM-1的水平随AGM剂量的增加而降低；给药剂量一定时，hRSV F mRNA和ICAM-1水平随给予AGM间隔时间的延长而升高，差异有统计学意义（ P＜0．05）。 KGM组内，给药剂量和给药时间对小鼠肺组织hRSV F mRNA和ICAM-1水平无明显影响（ P＞0．05）。结论 AGM能减轻hRSV感染小鼠肺组织的炎症，在小鼠体内具有抗hRSV的作用，且这种抗病毒作用具有剂量和时间依赖性。Objective To evaluate the anti-human respiratory syncytial viurs （ hRSV ） effect of aminoglucomannan （ AGM） in mice.Methods BALB/c mice infected with hRSV were randomly divided into AGM and konjac glucomannan （ KGM） groups with 54 in each.Then, mice in AGM group and KGM group were subdivided into three groups and treated with different doses of AGM or KGM as 2.5 μmol/L, 0.25 μmol/L or 0.025μmol/L.Each subgroup was further divided into 3 groups with 6 in each according to administration intervals of AGM or KGM as 8 h, 12 h or 24 h.All the mice are sacrificed at 72 h.The general condition of the mice was observed everyday.Reverse transcription-polymerase chain reaction （ RT-PCR） was used to detect the expression of hRSV fusion protein （ F） mRNA in the lung tissue of the mice. HE staining method and immunohistochemistry technique for intercellular cell adhesion molecule-1 （ ICAM-1） expression in the lung tissue were used to evaluate the inflammation in the lung tissue.ANOVA for randomized block design was used to examine the influence of dose and administration intervals on the expressions of hRSV F mRNA and ICAM-1.Results Reduced activity and asthma were observed in 16 mice in the KGM group, but not in the AGM group.And two mice in the KGM group died at d3, but there was no death in the AGM group.RT-PCR showed that the levels of hRSV F mRNA in lung tissues were 0.49 ＆#177;0.21 in AGM group and 0.88 ＆#177;0.06 in KGM group （t=6.71, P〈0.05）.HE staining of lung tissue showed that the inflammation in KGM group was more serious than that in AGM group.The expression of ICAM-1 in AGM group was much lower than that in KGM group, and there was statistical difference between two groups （t=13.88, P〈0.05）.In AGM group, the levels of RSV F mRNA and ICAM-1 decreased with the increase of AGM’s concentration and rised with longer administration intervals （P〈0.05）, but these were not observed in KGM group （P〉0.05）.Conclusion AGM can alleviate the inflammation of lung tissue i

关 键 词：甘露聚糖类 氨基葡甘露聚糖 呼吸道合胞病毒 抗病毒药 

分 类 号：R[医药卫生]