壬基酚分子印迹聚合物的制备及其识别特性的研究  

Study on preparation of nonylphenol molecular imprinted polymers and its recognition character

作　　者：孟磊[1] 苏惠[1] 韦朋[1] 袁志华[2] 赵仲麟[1] 马斌强[1] 袁超[1] 

机构地区：[1]河南农业大学理学院,河南郑州450002 [2]河南农业大学机电工程学院,河南郑州450002

出　　处：《河南农业大学学报》2016年第2期214-218,共5页Journal of Henan Agricultural University

基　　金：河南省教育厅科技研究重点项目(12A150012);郑州市科技攻关项目(083SGYG-24123-3)

摘　　要：为了分离测定环境雌激素壬基酚,本研究以壬基酚为模板分子,α-甲基丙烯酸为功能单体,乙二醇二甲基丙烯酸酯为交联剂,偶氮二异丁腈为引发剂制备了壬基酚印迹聚合物。采用动态和静态两种方法研究了该印迹材料对壬基酚的结合性能与分子识别特性。结果表明,印迹材料对模板分子壬基酚具有良好的特异性识别作用,经过Scatchard模型分析,壬基酚印迹聚合物上有两类不同性质的结合位点,2种结合位点的解离常数分别为1.99、32.05 m Lg^(-1),对壬基酚的最大表观吸附量分别为29.72、240.38μmol·g^(-1)。将该聚合物应用于固相萃取中,以鲤鱼~为样品的加标回收实验表明,此印迹聚合物可以对痕量壬基酚进行富集分离测定,且可重复使用。The molecularly imprinted polymers（ MIPs） were prepared by using nonylphenol（ NP） as template,α-methylacrylic acid as functional monomer,ethylene glycol dimethyl acrylate as the crosslinking agent,azobisisobutyronitrile as initiator,and toluene as solvent. Then the static and dynamic experiment results showed that MIPs had specific recognition selectivity and excellent binding selectivity for the template molecule. Scatchard analysis indicated that the nonylphenol imprinted polyer had two kinds of recognitionsites,the dissociation constant being 1. 99 and 32. 05 mL·g^-1,the apparent maximum absorption capacity being 29. 72 and 240. 38 μmol·g^-1,respectively. Besides,in a recovery experiment by adding standard samples with carp,the result shows that imprinted material of NP can enrich and separate for trace amounts of NP,and it is also easy to be used repeatedly.

关 键 词：分子印迹聚合物 壬基酚 固相萃取 

分 类 号：TS201[轻工技术与工程—食品科学]