HPV-DNA载量检测在宫颈病变诊断及预测中的意义  

Significance of human papillomavirus DNA load detection in the diagnosis and prediction of cervical neoplasia

作　　者：张慧慧[1] 韩丽丽[1] 王华杰[1] 付莉[1] 

机构地区：[1]吉林大学第二医院妇产科,吉林长春130041

出　　处：《中国实验诊断学》2016年第5期786-788,共3页Chinese Journal of Laboratory Diagnosis

摘　　要：目的探讨检测女性生殖道感染人乳头瘤病毒DNA(HPV-DNA)载量在宫颈病变诊断及预测中的意义。方法随机收集正常对照组120例,CINⅠ组60例,CINⅡ组60例,CINⅢ组60例、宫颈癌组60例,采用第二代杂交捕获实验(HC-Ⅱ)进行HPV-DNA检测,分析不同类型宫颈病变与HPV-DNA载量的关系。结果正常对照组HPV-DNA阳性率为58.33%,显著低于CINⅠ组(75%)、CINⅡ组(90%)、CINⅢ组(91.67%)及宫颈癌组患者(93.33%)(P<0.01)。正常对照组患者的HPV-DNA载量分级无显著的相关性(P>0.01),但CINⅠ-Ⅲ组及宫颈癌组患者的病变程度与HPV-DNA载量分级均具有明显的相关性(P<0.01);在低、中和高度载量中,各级宫颈病变的OR值分别是CINⅠ组为1.27、2.17和6.67,CINⅡ组为1.19、9.58和26.04,CINⅢ组为1.19、8.50和41.25,宫颈癌组为0.89、6.25和67.19,结果表明CINⅠ-Ⅲ组及宫颈癌组OR值均呈现出随着HPV-DNA载量级别增高风险增高的趋势。结论 HPV-DNA病毒载量越高,发生宫颈癌及宫颈癌前病变的风险性就越大。提示我们要警惕高载量HPV的持续感染,预防宫颈癌前病变及宫颈癌的发生。Objective To investigate and detect the human papillomavirus DNA（HPV-DNA）load in the cervix of the female genital tract infection,which is significant in diagnosis and prediction of cervical neoplasia.Methods In normal control group,120 cases were collected,60 cases in CINⅠgroup,60 cases in CINⅡ group,60 cases in CINⅢ group and 60 cases in cervical cancer group.HPV-DNA was detected by the Hybrid CaptureⅡ（HC-Ⅱ）system,and to analysis the relationship between different types of cervical neoplasia and HPV-DNA load.Results The positive rate of HPV-DNA in normal control group was 58.33%,which was significantly lower than that of CINⅠgroup（75%）,CINⅡ group（90%）,CINⅢ group（91.67%）and cervical cancer group（93.33%）（P〈0.01）.There was no significant correlation between HPV-DNA load levels in normal control group（P〉0.01）,but CINⅠ group,CINⅡ group,CINⅢgroup and cervical cancer were significantly correlated with HPV-DNA（P〈0.01）;At low,medium and high loads,the odds ratio（OR）values of different levels of cervical neoplasia were CINⅠgroup：1.27,2.17 and 6.67,CINⅡgroup：1.19、9.58 and 26.04,CINⅢgroup：1.19,8.50 and 41.25,the results showed that the OR values of CINⅠ-Ⅲ groups and cervical cancer group showed an increasing trend with the incre-ase of HPV-DNA loading level.Conclusion The higher HPV-DNA viral load,the higher the risk of cervical cancer and cervical intraepithelial neoplasia may be greater,which prompted us to be wary of high load HPV persistent infection,prevention of cervical intraepithelial neoplasia and cervical cancer.

关 键 词：人乳头瘤病毒 宫颈上皮内瘤变 宫颈癌 相关性 

分 类 号：R737.33[医药卫生—肿瘤]