胍丁胺对大鼠应激性体温升高的抑制作用  

Inhibitory effects of agmatine on stress-induced hyperthermia in rats

作　　者：熊资 杨永录[1] 胥建辉[1] 卜舒[1] 罗蓉[1] 陈冰[1] 

机构地区：[1]成都医学院体温与炎症四川省高校重点实验室,成都610500

出　　处：《中国应用生理学杂志》2016年第3期270-273,共4页Chinese Journal of Applied Physiology

基　　金：四川省教育厅科研基金资助项目(12ZB204;15ZA0251)

摘　　要：目的：观察胍丁胺（AGM）是否能降低或反转应激性体温过高反应。方法：61只雄性SD大鼠随机分为3部分,每部分再分为对照组和AGM组。在实验过程中,人工气候箱和开放实验箱内的温度均保持在22℃。1用无线遥测技术连续测量大鼠的体温和活动,观察腹腔注射AGM对安静状态下大鼠正常体温和活动的影响（n=8）;2将大鼠放置在开放实验箱中60 min复制应激性体温过高的模型,用无线遥测技术连续测量开放实验箱内大鼠体温和活动的变化（n=7~8）;3用美国哥伦布公司动物代谢分析系统测量AGM对大鼠能量代谢的影响（n=7）。结果：1腹腔注射AGM 80 mg/kg能引起正常大鼠出现明显低温反应（-0.46±0.11）℃,而注射AGM 40 mg/kg则对正常体温无明显影响。2对照组大鼠腹腔注射生理盐水后,置于开放实验箱内体温升高达（0.78±0.16）℃;给大鼠注射AGM 40或80 mg/kg后,置于开放实验箱内60 min时,体温则分别降低（0.34±0.11）℃和（0.81±0.14）℃。3AGM 80 mg/kg能明显降低大鼠的耗氧量和产热量。结论：AGM能引起正常大鼠出现低温反应和明显翻转应激性体温升高反应,其作用可能与AGM能降低能量代谢有关。Objective： To examine whether agmatine（AGM） would alter stress-induces hyperthermic response. Methods： Sixtyone male SD rats were randomly divided into three experiments. Each experiment was divided into control group and AGM group. During the experiments,the animals were maintained in a chamber at 22℃. 1Effects of intraperitoneal injecting 40 or 80 mg / kg AGM on normal core temperature and activity were observed in undisturbed rats using radiotelemetry（n = 8）. 2Stress-induced hyperthermia model was established by placing rats in an open-field chamber for 60 min. Rats were dosed intraperitoneally with AGM or saline,and placed immediately inside the open-field chamber. Core temperature and motor activity were monitored by radiotelemetry in an open-field chamber（ n = 7 ~ 8）.3Effect of AGM on energy metabolism was measured by Columbus Oxymax Lab Animal Monitoring System（n = 7）. Results： 1Rats administered with 80 mg / kg AMG showed significant hypothermic responses（-0. 46 ± 0. 11） ℃,while 40 mg / kg AMG had no significant effect on the normal core temperature. 2Core temperature of control group increased by（0. 78 ± 0. 16） ℃ during open-field exposure,whereas rats administered 40 and 80 mg / kg AGM underwent a（0. 34 ± 0. 11） ℃ and（0. 81 ± 0. 14） ℃ reductions in core temperature within 60 min,respectively. 3Oxygen consumption and energy metabolism were significantly reduced by AGM（80 mg / kg）.Conclusion： The data demonstrated that AGM induced hypothermic responses in rats and reversed stress-induced hyperthermia,and its effect might attribute to the suppression of energy metabolism.

关 键 词：胍丁胺 应激性体温过高 低温反应 能量代谢 大鼠 

分 类 号：R364.6[医药卫生—病理学]