长链非编码RNA LOC100294362对乳腺癌细胞增殖和侵袭的影响  

Effect of Long Non-coding RNA LOC100294362 on Proliferation and Invasion of Breast Cancer Cells

作　　者：奉水东[1] 朱责梅 杨丝丝[2] 何剑琴[2] 张恺芳[2] 阳际花 凌宏艳[2] 

机构地区：[1]南华大学社会医学与卫生事业管理学教研室,衡阳421001 [2]南华大学生理学教研室,衡阳421001

出　　处：《肿瘤防治研究》2016年第5期366-370,共5页Cancer Research on Prevention and Treatment

基　　金：教育部留学归国基金(教外司留[2014]1685号);中国博士后科学基金(2012M511384);湖南省科技厅重点项目(2015JC3085);南华大学研究生科研创新项目(2015XCX36)

摘　　要：目的探讨长链非编码RNA LOC100294362在乳腺癌组织及细胞系中的表达情况,观察LOC100294362对乳腺癌MCF-7细胞增殖和侵袭的影响,并探索其可能的作用机制。方法采用定量反转录PCR(q RT-PCR)方法检测乳腺癌组织及细胞系中LOC100294362的表达水平;MCF7细胞转染过表达或干扰LOC100294362后,采用MTT法和Transwell小室法检测上调或沉默LOC100294362对MCF-7细胞增殖和侵袭能力的影响,Western blot法检测p53蛋白表达。结果与正常组织及细胞相比,LOC100294362在乳腺癌组织和细胞中显著高表达;过表达LOC100294362导致MCF-7细胞增殖和侵袭能力增加,p53蛋白表达降低;沉默LOC100294362导致MCF-7细胞增殖和侵袭能力受到抑制,p53蛋白表达升高。结论 LOC100294362可能通过抑制p53蛋白的表达从而促进乳腺癌细胞的增殖和侵袭。Objective To investigate the expression level of long non-coding RNA LOC100294362 in breast cancer tissues and cell lines, to observe the effect of LOC100294362 on the proliferation and invasion of breast cancer MCF-7 cells and to explore its possible mechanisms. Methods Quantitative reverse-transcription PCR（q RT-PCR） was performed to detect the relative expression of LOC100294362 in breast cancer tissues and cell lines. After MCF-7 cells were transfected with over-expressed or si RNA-LOC100294362, the effect of up-regulated or down-regulated LOC100294362 on MCF-7 cells proliferation and invasion were detected by MTT assay and Transwell assay, and the expression level of p53 protein was tested by Western blot assay. Results Compared with their corresponding normal tissues and cell lines, LOC100294362 was significantly highly expressed in breast cancer samples and cell lines. Over-expressed LOC100294362 enhanced MCF-7 cells proliferation and invasion abilities, and decreased the expression of p53 protein; however, silencing LOC100294362 inhibited MCF-7 cells proliferation and invasion abilities, and increased the expression of p53 protein. Conclusion LOC100294362 promotes MCF-7 cells proliferation and invasion probably by inhibiting the expression of p53 protein.

关 键 词：长链非编码RNA LOC100294362 MCF-7细胞 增殖 侵袭 P53 

分 类 号：R781.5[医药卫生—口腔医学]