基于发光细菌的细颗粒物(PM_(2.5))可溶性提取液综合生物效应测试方法及其应用  

Application of Luminescent Bacteria in the Acute Toxicity Test of the Water-Soluble Constituents of PM_(2.5)

作　　者：孙成华[1,2] 石爱军[1,2] 刘保献[1,2] 张大伟[1,2] 陈添[3] 刘建武[4] 刘康[1,2] 周健楠[1,2] 洪姗姗[1,2] 

机构地区：[1]北京市环境保护监测中心,北京100048 [2]大气颗粒物监测技术北京市重点实验室,北京100048 [3]北京市环境保护局,北京100048 [4]北京师范大学核科学与技术学院,北京100875

出　　处：《生态毒理学报》2016年第1期353-361,共9页Asian Journal of Ecotoxicology

基　　金：北京市环境保护监测中心自筹经费课题(2014-017)

摘　　要：为科学评估PM_(2.5)对生物体综合生物效应,研究建立了利用费氏弧菌检测PM_(2.5)水溶性提取液的毒性测试方法,确立了PM_(2.5)样品提取液发光细菌毒性测试实验质量控制办法。对春节烟花爆竹燃放和沙尘污染过程的PM_(2.5)实样测试表明:烟花爆竹燃放期间的PM_(2.5)样品提取液发光抑制率值与微量金属元素等有毒有害组分浓度显著相关;沙尘污染期间的PM_(2.5)样本提取液中地壳元素浓度和发光抑制率值显著不相关。The fine particulate matter（ PM2.5） can be breathed into lungs and threatens human health. For scientific assessment of the comprehensive biological effects of PM2.5,a simple method with the laboratory quality control standards for testing the toxicity of the PM2.5water-soluble components was established by using Vibrio fischeri as an indicator bacterium. A series of toxicity tests performed on this luminescent bacterium were carried out with thePM2.5samples from the Spring Festival fireworks and the dust pollution,respectively. Our results showed that the luminous inhibition rate had a significant correlation with the concentrations of poisonous and harmful trace metal elements discharged by the fireworks and crackers during the Spring Festival,whereas the luminous inhibition rate was not associated with the concentrations of the earth＇s crust elements in the PM2.5extracting solution.

关 键 词：发光细菌 生物毒性测试 发光抑制率 PM2.5 

分 类 号：X171.5[环境科学与工程—环境科学]