人类脱嘌呤/脱嘧啶核酸内切酶1在三阴性乳腺癌中的表达研究  

Expression of APE-1 in Triple Negative Breast Cancer

作　　者：陈天然[1] 应明真[1] 何妙霞[2] 刘传[1] 王雅杰[1] 

机构地区：[1]第二军医大学附属长海医院肿瘤科,200433 [2]第二军医大学附属长海医院病理科,200433

出　　处：《医学研究杂志》2016年第5期19-23,179,共6页Journal of Medical Research

基　　金：国家自然科学基金资助项目(81372854;81102010);上海市科委基础研究重点项目(13NM1401504);上海市科委医学引导项目(15411966200);上海市重点学科建设项目(B905);第二军医大学附属长海医院1255学科建设特色培育项目(CH125530400)

摘　　要：目的检测人类脱嘌呤／脱嘧啶核酸内切酶1（apurinic／apyrimidinic endonuclease／redox effector factor1，APE-1／REF-1）在乳腺癌中的表达，分析其与乳腺癌的临床病理特征的关系，及APE-1对三阴性乳腺癌患者预后的影响。方法采用免疫组化Envision两步法检测323例乳腺癌中APE-1蛋白的表达，其中108例为三阴性乳腺癌。计数资料采用，检验和Fisher精确检验。采用Kaplan—Meier法进行生存分析及Log—rank检验进行生存率的比较，并用COX比例风险回归模型进行多因素分析。结果APE-1在乳腺癌组织较癌旁组织有更高的表达，且在非三阴性乳腺癌中与肿瘤大小、腋窝淋巴结状态、临床分期、雌激素受体、人类表皮生长因子2型受体的表达显著相关（P〈0．05）。在三阴性乳腺癌中，APE-1表达与肿瘤大小（P=0．006）呈正相关，且APE-1低表达组有较好的生存率（P=0．007）。腋窝淋巴结阳性的三阴性乳腺癌患者中，APE一1低表达组有较好的预后（P=0．042）。结论APE一1是影响三阴性乳腺癌患者预后的独立因子，可能成为三阴性乳腺癌的潜在治疗靶点。Objective To investigate the expression of Apurinic/apyrimidinic endonuclease 1 （Apurinic/apyrimidinic endonucle- ase/redox effector factor 1, APE - 1/REF - 1 ） in breast cancer. We analyzed the relationship between APE - 1 and clinicopathological factors in breast cancer and investigate the prognostic value of APE - 1 in triple negative breast cancer （TNBC） patients. Methods Immunohistochemistry with two - step Envision system was used to detect the APE - 1 expression in 323 eases of breast cancer, including 108 cases of TNBC. Data were analyzed by chi - square test and Fisher＇s exact test. Kaplan - Meier survival analysis method and the Logrank test were undertaken in survival and COX proportional hazards regression model was used for multivariate analysis. Results Over - expression of APE - 1 was found in breast cancer tissue compared with adjacent tissues. In non - triple - negative breast cancer, APE - 1 was correlated to tumor size, axillary lymph node metastasis, clinical stage, estrogen receptor （ER） , and human epidermal growth factor receptor- 2（HER- 2）（P 〈 0.05 ）. In triple -negative breast cancer, the expression of APE- 1 were positively correlated with tumor size （ P = 0. 006） and the patients with low expression of APE - 1 （ P = 0. 007） have better survival rate. In axillary lymph node metastasis patients of TNBC, low expression of APE - 1 group （P = 0. 042） had a better prognosis. Conclusion APE - 1 is an independent prognostic factor in TNBC and it could become a new potential therapeutic target of TNBC.

关 键 词：乳腺肿瘤 人类脱嘌呤/脱嘧啶核酸内切酶1 三阴性乳腺癌 

分 类 号：R73[医药卫生—肿瘤]