3种血清指标联合检测对肝硬化合并细菌性腹膜炎的诊断价值  

The clinical significance of combined detection of three kinds of serum indexes for liver cirrhosis patients with bacterial peritonitis

作　　者：李惠平[1] 蔡伟雄[1] 徐锦芳[1] 茹文渊[1] 

机构地区：[1]惠州市博罗县人民医院检验科,广东惠州516100

出　　处：《国际检验医学杂志》2016年第10期1375-1376,1379,共3页International Journal of Laboratory Medicine

摘　　要：目的探讨血清中性粒细胞防御素(HNP)、血清降钙素原(PCT)和单核细胞趋化蛋白-1(MCP-1)联合检测在肝硬化合并细菌性腹膜炎患者中的临床价值。方法将受试者分为肝硬化合并细菌性腹膜炎(SBP组)、无细菌性腹水组(非SBP组)和对照组,采用电化学发光免疫分析法检测血清HNP水平,免疫荧光定量法检测血清PCT和酶联免疫吸附法检测血清MCP-1水平,分析联合检测与单项检测的敏感度和特异度,同时分析SBP组患者经抗菌药物有效治疗后其血清HNP、PCT及MCP-1水平变化。结果 SBP组血清HNP、PCT及MCP-1水平均显著上升,与非SBP组和对照组对比,差异均有统计学意义(P<0.05);经治疗后,三者水平均显著下降;HNP、PCT及MCP-1联合检测的敏感度和特异度高于单项检测,差异均有统计学意义(P<0.05)。结论联合检测HNP、PCT及MCP-1对于肝硬化合并细菌性腹膜炎患者的早期诊断和疗效观察具有重要的临床意义。Objective To investigate clinical significance of the combined detection of levels of HNP,PCT and MCP-1in ascites of liver cirrhosis patients with spontaneous bacterial peritonitis.Methods Patients were divided into 3groups,SBP group,non-SBP group and healthy group.The level of HNP was detected by electrochemiluminescent immunoassay,and the levels of PCT and MCP-1were detected by enzyme-linked immuno sorbent assay（ELISA）.The sensitivity and specificity of combined detection were analyzed.Then the changes of HNP,PCT and MCP-1were also analyzed after treatment in SBP group.Results There were significant difference in the serum levels of HNP,PCT and MCP-1between SBP group and non-SBP group（P〈0.05）.And after treatment,the serum levels of HNP,PCT and MCP-1were all declined.The sensitivity and specificity of combined detection were significantly higher than single detection（P〈0.05）.Conclusion Combined detection of HNP,PCT and MCP-1is helpful for liver cirrhosis patients with spontaneous bacterial peritonitis in early diagnosis and monitoring therapy.

关 键 词：细菌性腹膜炎 血清中性粒细胞防御素 降钙素原 细胞趋化蛋白1 联合检测 

分 类 号：R575.2[医药卫生—消化系统]