IL-21通过Notch信号调控人牙周膜细胞RANKL表达和增殖的研究  

IL-21 Regulates RANKL Expression and Proliferation Activity in Human Periodontal Ligament Cells via Notch Signal Pathway

作　　者：邢泉[1] 秦伟[1] 张晓磊[1] 樊明文[2] 

机构地区：[1]中山大学光华口腔医学院附属口腔医院,广东省口腔医学重点实验室,广东广州510055 [2]武汉大学口腔医学院牙体牙髓科,湖北省口腔基础医学重点实验室-省部共建国家重点实验室培育基地口腔生物医学教育部重点实验室,湖北武汉430079

出　　处：《口腔医学研究》2016年第5期460-463,469,共5页Journal of Oral Science Research

基　　金：国家自然科学基金项目(编号:81300874);广东省医学科研基金项目(编号:B2013152,A2014255)

摘　　要：目的:观察IL-21对人牙周膜成纤维细胞RANKL/OPG的表达以及增殖活性的影响,以及Notch信号通路在其中的作用,探讨IL-21在根尖周炎骨破坏中的作用机制。方法:使用不同浓度(0、10、50、100μg/L)的IL-21作用于人牙周膜成纤维细胞,并选择最佳刺激浓度IL-21(50μg/L)处理人牙周膜成纤维细胞,观察不同的时间点(0、12、24h),通过实时定量PCR和ELISA的方法检测细胞中RANKL/OPG的表达水平。同时使用实时定量PCR和Western-blot检测IL-21对人牙周膜成纤维细胞Notch1表达水平的影响。运用Notch信号的阻断剂GSI(5μmol/L)预处理人牙周膜成纤维细胞后,再检测IL-21对人牙周膜成纤维细胞RANKL/OPG的表达水平的影响。CCK8法测定各个实验组细胞增殖活性。结果:IL-21可显著提高人牙周膜成纤维细胞RANKL的表达水平,对OPG的表达无显著影响。IL-21显著抑制人牙周膜成纤维细胞的增殖活性。结论:IL-21通过Notch信号通路上调人牙周膜成纤维细胞的RANKL表达,抑制其增殖活性。Objective：To explore the effect of interleukin-21（IL-21）via Notch signal pathway on the expression of RANKL/OPG and proliferation in hPDLCs and to investigate the possible role of IL-21 in bone resorption of apical periodontitis.Methods：hPDLCs were treated with IL-21 in different concentrations（0,10,25,50μg/L）.Then the optimal concentration（50μg/L）was used in the time-dependent experiment.The expressions of RANKL/OPG were detected by real-time PCR and ELISA.The expressions of RANKL/OPG were detected by real-time PCR and ELISA.Real-time PCR and western-blot were conducted to detect the expression of Notch1 in hPDLCs treated by IL-21.After pretreatment with GSI（5μmol/L）to inhibit Notch1 signaling pathway,the expressions of RANKL/OPG of hPDLCs treated with IL-21（50μg/L）were investigated.CCK8 was carried out to detect the proliferation activity in the different groups of hPDLCs.Results：The expressions of RANKL were significantly up-regulated by IL-21.IL-21 decreased the cell proliferation activity in hPDLCs.Conclusion：IL-21up-regulated the expression of RANKL and reduced proliferation activity in hPDLCs via Notch signal pathway.

关 键 词：IL-21 NOTCH 人牙周膜成纤维细胞 RANKL 增殖 

分 类 号：R781.4[医药卫生—口腔医学]