NSCL/P患者中异常表达microRNA与WNT家族的关联性研究  

Correlation Between Abnormal MicroRNA Expression with Wnt Family in NSCL/P Patients

作　　者：王鑫[1] 王姗[1] 孟琰[1] 强冬霞 施磊[1] 苏艳国 赵尔杨[1] 

机构地区：[1]哈尔滨医科大学第一临床医学院.口腔医学院口腔病理科,黑龙江哈尔滨150001

出　　处：《口腔医学研究》2016年第5期470-473,共4页Journal of Oral Science Research

基　　金：全国"重生行动"李嘉诚科研基金项目(无项目编号)

摘　　要：目的:探讨非综合征性唇腭裂患者中microRNA表达谱差异及显著改变microRNA与WNT家族的关联性。方法:采用涵盖当前miRbase数据库中所有人类microRNA的miRCURY^(TM)探针的基因芯片对4例非综合征性唇腭裂患者的唇腭组织与4例正常新生儿脐带组织所提取的microRNA进行分析研究,并对差异性表达显著的microRNA进行生物信息学分析。结果:共筛选出254个与非综合征性唇腭裂相关的差异表达microRNA:181个在非综合征性唇腭裂组织中表达上调,73个表达下调(P<0.05)。根据差异倍数与P值大小,选取十个表达差异较大的microRNAs进行生物信息学靶基因预测,结果显示hsa-miR-1260b,hsa-miR-205-5p,hsa-miR-24-3p,hsa-miR-27b-3p和hsa-miR-720均靶向结合于Wnt5a、Wnt9b、Wnt10a等Wnt家族的信号分子。结论:差异表达较显著的microRNA通过与Wnt家族信号分子靶向结合参与调控唇腭裂的发育过程。Objective：To investigate the miRNA expression in nonsyndromic cleft lip patients with or without cleft palate and its correlation with Wnt family in nonsyndromic cleft lip patients with or without cleft palate.Methods：Gene chips of MiRCURYTM probe were used to detect and compare the microRNA expression of the lip and palate tissues in 4NSCL/P patients as well as umbilical cord tissues in 4normal newborns.Results：In total,254 differentially expressed miRNAs were detected,among which 181up-regulate and 73down-regulate（P〈0.05）.According to the fold change and P value,the top ten different miRNAs were selected for target prediction.The results showed that hsa-miR-1260 b,hsa-miR-205-5p,hsa-miR-24-3p,hsa-miR-27b-3p and hsamiR-720 are combined with Wnt5 a,Wnt9band Wnt10 a.Conclusion：Differentially expressed miRNA may control the development of lip and palate by targeting Wnt family in embryonic period.

关 键 词：微小核糖核酸 非综合征性唇腭裂 生物信息学分析 WNT家族 

分 类 号：R782.2[医药卫生—口腔医学]