Enzyme-assisted Extraction and Enrichment of Galanthamine from Lycoris aurea  

作　　者：Chun-lian Tian Peng Wang Ji-xin Qin Xiao-pan Liu Ke Song Zhuo-bing Xiao 

机构地区：[1]Key Laboratory of Forest Products and Chemical Industry Engineering,Jishou University,Zhangjiajie 427000,China [2]The First High School of Zhangjiajie,Zhangjiajie 427000,China

出　　处：《Chinese Herbal Medicines》2016年第2期182-188,共7页中草药（英文版）

基　　金：Hunan Provincial Science and Technology Department(2012TP4002-3);Zhangjiajie Science and Technology Bureau(2014YB17)

摘　　要：Objective To explore the optimum condition for complex enzyme-assisted extraction of galanthamine from Lycoris aurea by L_9（3~4） orthogonal array design and separation effect of cation exchange resin on galanthamine. Methods Cellulase and pectinase solution was used as the extraction solvent. The effects of p H value of enzyme, amount of complex enzyme, dissociation time, and enzymatic hydrolysis temperature on the extraction results were investigated. Results The optimal conditions were obtained as follows： ratio of solid to liquid（g：mL） 1：10, pH value 4.5, amount of complex enzymes 4%, enzymatic hydrolysis temperature 50oC, and reaction time 2.0 h. Under these conditions, the extraction yield of galanthamine was 0.0294%. In addition, D-001 cation exchange resin was selected for separation of galanthamine. The separation conditions were that adsorption flow rate was 3 BV/h with reagent of pH2 and the desorption flow rate was 3 BV/h with 70% ethanol solution containing 1.5 mol/L ammonia. After separation, the content of galanthamine was increased to 12.31%. Conclusion The results provide a reference for industrial production of galanthamine.Objective To explore the optimum condition for complex enzyme-assisted extraction of galanthamine from Lycoris aurea by L_9（3~4） orthogonal array design and separation effect of cation exchange resin on galanthamine. Methods Cellulase and pectinase solution was used as the extraction solvent. The effects of p H value of enzyme, amount of complex enzyme, dissociation time, and enzymatic hydrolysis temperature on the extraction results were investigated. Results The optimal conditions were obtained as follows： ratio of solid to liquid（g：mL） 1：10, pH value 4.5, amount of complex enzymes 4%, enzymatic hydrolysis temperature 50oC, and reaction time 2.0 h. Under these conditions, the extraction yield of galanthamine was 0.0294%. In addition, D-001 cation exchange resin was selected for separation of galanthamine. The separation conditions were that adsorption flow rate was 3 BV/h with reagent of pH2 and the desorption flow rate was 3 BV/h with 70% ethanol solution containing 1.5 mol/L ammonia. After separation, the content of galanthamine was increased to 12.31%. Conclusion The results provide a reference for industrial production of galanthamine.

关 键 词：cation exchange resin enzyme-assisted extraction GALANTHAMINE Lycoris aurea SEPARATION 

分 类 号：R284[医药卫生—中药学]