乌鳢和斑鳢EST序列微卫星信息分析  

Analysis of simple sequence repeats information in expressed sequence tags of Channa argus and Channa maculatus

作　　者：谢楠[1] 刘凯[1] 冯晓宇[1] 姚桂桂[1] 潘彬斌[1] 

机构地区：[1]杭州市农业科学研究院,杭州310024

出　　处：《淡水渔业》2016年第3期16-22,共7页Freshwater Fisheries

基　　金：浙江省水产农业新品种选育重大科技专项(2012C12907-7)

摘　　要：乌鳢（Channa argus,♂）、斑鳢（Channa maculatus,♀）是杂交鳢＂杭鳢1号＂的亲本。利用高通量测序技术对乌鳢和斑鳢的肝脏进行转录组测序获得大量EST序列后,利用MISA软件进行微卫星信息分析,结果表明,通过转录组测序获得乌鳢EST序列59 959条,长度45 mb,发现15 428个SSR,出现频率为25.73%;获得斑鳢EST序列44 337条,长度27 mb,发现8 439个SSR,出现频率为19.03%。在乌鳢和斑鳢EST-SSR中,重复单元以1～2碱基重复为最多,并以长度小于16 bp的短重复序列为主,间隔SSR和复合SSR的EST序列RPKM均值要低于单纯型SSR的EST序列RPKM均值,且在单纯型SSR中SSR长度越长,其RPKM均值则越低。In this experiment,a large number of Expressed Sequence Tags（ EST） were obtained from transcriptome sequences of livers in Channa argus and Channa maculatus by high-throughput sequencing technology,and Simple Sequence Repeats（ SSR） Information was analysed. The result showed that,59 959 EST with 45 mb length were obtained from Channa argus,and 15428 EST-SSR were mined out by MISA,with the frequency of 25. 73%; while 44 337 EST with27 mb length were obtained from Channa maculatus,and 8 439 EST-SSR were mined out by MISA,with the frequency of19. 03%. In the EST-SSR of C. argus and C. maculatus,nucleotide and dinucleotide repeats were the main types,while the repeats less than 16 bp in length were the main types. Mean values of reads per kilobase per million（ RPKM） of EST in both interrupted and compound SSR were lower than mean values of RPKM of EST in pure SSR,and in the pure EST-SSR,the longer SSR in length,the lower mean values of RPKM.

关 键 词：乌鳢(Channa argus) 斑鳢(Channa maculatus) EST序列 微卫星 

分 类 号：S917.4[农业科学—水产科学]