SOX2基因在胶质瘤中的表达及生物学作用  

Expression of SOX2 gene and its biological role in human gliomas

作　　者：常英男[1] 王策[1] 赵天书[1] 

机构地区：[1]哈尔滨医科大学附属第四医院神经外科,哈尔滨150006

出　　处：《中国临床神经外科杂志》2016年第5期270-273,共4页Chinese Journal of Clinical Neurosurgery

基　　金：黑龙江省科技厅课题(D201140)

摘　　要：目的探讨SOX2基因在人胶质瘤中的表达水平、甲基化状态以及生物学作用。方法选取人胶质瘤组织标本108例,正常脑组织30例。采用PCR的方法检测SOX2基因的表达水平和甲基化状态。MTT和软琼脂集落形成试验检测SOX2对胶质瘤U87和U251细胞的生物学作用。结果 SOX2在正常脑组织中的相对表达水平明显低于胶质瘤组织(P<0.01)。SOX2基因启动子区非甲基化比率在胶质瘤中分别为:Ⅰ级23.5%(4/17),Ⅱ级34.8%(8/23),Ⅲ级51.4%(19/37),Ⅳ级64.5%(20/31),高级别组(57.4%)与低级别组(30.0%)相比具有统计学差异(P<0.01)。过量表达SOX2基因可促进胶质瘤U87和U251细胞增殖和集落形成能力。结论 SOX2基因在胶质瘤组织高表达,其在胶质瘤中的表达水平可能受启动子区域甲基化状态影响,SOX2基因影响胶质瘤的肿瘤学特性。Objective To explore the expression level, methylation status and biological role of SOX2 in human gliomas. Methods The expression levels and the methylation status of SOX2 were determined by PCR technique in 108 specimens of glioma tissues and 30 specimens of normal brain tissues. The roles of SOX2 in the tumor characteristics of glioma cell lines were evaluated by MTF and colony-formation assay. Results The expression level （0.49±0.2） of SOX2 was significantly higher in the glioma tissues than that （0.154± 0.076） in the normal brain tissues （P〈0.01）. The methylation ratio （42.6%, 29/68） of SOX2 was significantly lower in the high grade （grades Ⅱ - Ⅲ ） glioma tissues than that （70.0%, 28/40） in the low grade （grades Ⅰ - Ⅱ ） glioma tissues （P〈0.01）, which was significantly lower than that （100.0%） in the normal brain tissues （P〈0.05）. MTT and colony-formation assay showed that SOX2 could promote ghoma cells proliferation and anchorage independent growth capacities （P〈0.05）. Conclusions Upregulation of SOX2 expression was found in the human gliomas tissues. The expression of SOX2 was influenced by its promoter methylation status. Overexpression of SOX2 in glioma cell lines can promote glioma cells proliferation.

关 键 词：胶质瘤 SOX2基因 甲基化 肿瘤学特性 

分 类 号：R739.41[医药卫生—肿瘤] Q786[医药卫生—临床医学]