大豆GmPLC2基因的序列分析及表达特性  

Sequence Analysis and Expression Characterization of Soybean GmPLC2 Gene

作　　者：邓宇[1] 王骐[2] 董金晔 高红桃[1] 王南[3] 王法微[3] 李海燕[1,3] 

机构地区：[1]吉林农业大学生命科学学院,长春130118 [2]东北师范大学附属中学,长春130021 [3]吉林农业大学生物反应器与药物开发教育部工程研究中心,长春130118

出　　处：《吉林农业大学学报》2016年第2期138-144,共7页Journal of Jilin Agricultural University

基　　金：国家自然科学基金项目(31201144;31271746;31101091);教育部高等学校博士学科点专项科研基金项目(20122223120003);吉林省发改委项目(JF2012C002-04)

摘　　要：提取大豆叶片总RNA,利用RT-PCR法克隆GmPLC2基因的全长序列;利用生物信息学软件分析GmPLC2的蛋白三维结构及氨基酸组成、构建系统发育树;同时,利用实时定量PCR方法分析在不同逆境胁迫下GmPLC2基因的表达模式。结果表明:从大豆中克隆得到的GmPLC2基因全长1779bp,编码592个氨基酸。GmPLC2基因与绿豆、红车轴草的PLC基因编码氨基酸相似性为84.29%和79.63%。荧光定量PCR分析发现,盐碱、盐、碱、干旱和ABA胁迫处理后大豆叶片中GmPLC2基因的表达量出现不同程度的升高,碱胁迫6h时GmPLC2基因的表达量为0h的4倍。Total RNA was extracted from soybean leaves and full-length sequence of GmPLC2 was cloned by RT-PCR method. By using bioinformatics softwares, three-dimensional structure and ami- no acid compositions of proteins were analyzed and a phylogenetic tree was built. Meanwhile, ex- pression patterns of GmPLC2 gene under various abiotic stresses were investigated by real-time quantitative PCR method. The results indicate that GmPLC2 gene has a full-length of 1 779 bp and it encodes 592 amino acids. Amino acids encoded by GmPLC2 gene has a genetic similarity of 84.29% and 79.63% respectively with PLCs from Vigna radiate and Trifolium pretens, qRT-PCR analysis shows that expression levels of GmPLC2 gene rise under salt-alkali, salt, alkali, drought and ABA treatments. Expression level of GmPLC2 gene under alkali stress increases nearly fourfold compared to the control. The study presented in this paper will lay a foundation for further studies on functions of GmPLC2.

关 键 词：大豆 GmPLC2 基因表达 序列分析 

分 类 号：Q786[生物学—分子生物学] S565.1[农业科学—作物学]