钛表面人工肽抑制格登链球菌生长的实验研究  

Effects of the synthetic peptide coatings inhibiting the growth of Streptococcus gordonii on titanium surface

作　　者：马勍[1] 张溪[1] 张旭[1] 孙迎春[1] 高平[1] 

机构地区：[1]天津医科大学口腔医学院修复科,300070

出　　处：《天津医药》2016年第6期708-712,807,共5页Tianjin Medical Journal

基　　金：国家自然科学基金资助项目(31470919);天津市应用基础及前沿技术研究计划面上项目(14JCYBJC29600;13JCYBJC41300)

摘　　要：目的检测自主设计合成的人工肽在钛表面的吸附情况及吸附后的抗菌效果,为种植体抗菌的实验研究提供新的思路。方法使用Ex PASy Prot Param、Prot Scale软件、圆二色光谱和Zeta电位仪等,分析或测定人工肽与抗菌肽的各项理化性质及结构特征。利用常温孵育的方法将人工肽锚定于钛表面。使用X射线光电子能谱与原子力显微镜检测人工肽在钛表面的吸附。通过共聚焦激光显微镜观察锚定于钛表面的人工肽对格登链球菌的抑菌作用。使用透射电镜检测游离人工肽与抗菌肽对格登链球菌结构的破坏作用。结果自主设计合成的人工肽仍具有抗菌肽发挥抗菌作用所需的各项理化性质及结构特征。在孵育于5 g/L人工肽的PBS溶液中的钛片表面即可检测到人工肽的吸附,且该试件对格登链球菌的存活及黏附均有一定的抑制效果。结论自主设计合成的人工肽实现了在钛表面吸附并抑制格登链球菌生长的设想。Objective To detect the independently designed synthetic peptide adsorbed to the titanium surface and its inhibitory effect on streptococcus gordonii, and to provide a new means for antibiosis reseach on oral implants. Methods The physical and chemical properties of the synthetic peptide and antimicrobial peptide were measured by ExPASy Prot?Param tool, ProtScale analysis, circular dichroism and Zeta potential instrument. The synthetic peptide was anchored on the surface of the titanium specimen through incubation at room temperature. The adsorption of the synthetic peptide to the titani?um surface was examined by X-ray photoelectron spectroscopy (XPS) and the atomic force microscope (AFM). The inhibitory effect on streptococcus gordonii of the synthetic peptide fixed on the titanium surface was viewed by confocal laser scanning microscopy (CLSM). The destructive effects of the synthetic peptide and the antimicrobial peptide on streptococcus gordonii were observed through the transmission electron microscope (TEM). Results The independently designed synthetic peptide still had the physical and chemical properties that the antimicrobial peptide desired. The synthetic peptide had already been detected on the titanium surface after incubated in a 5 g/L synthetic peptide solution. The titanium specimen fixed with the synthetic peptide inhibited the survival and adhesion of streptococcus gordonii. Conclusion It suggests that the indepen?dently designed synthetic peptide might have reached the goal of bacterial inhibition on the titanium surface.

关 键 词：钛 种植体周围炎 格登链球菌 无机结合肽 抗菌肽 

分 类 号：R783.1[医药卫生—口腔医学]