血管平滑肌细胞凋亡在高血压脑出血中的作用  

The Role of Vascular Smooth Muscle Cell Apoptosis in Hypertensive Intracerebral Hemorrhage

作　　者：席杰[1] 张银辉[1] 张禅那[1] 宋莉[1] 宋燕[1] 惠汝太 陈敬洲[1] 

机构地区：[1]北京协和医学院,中国医学科学院,国家心血管病中心,阜外医院,心血管疾病国家重点实验室,北京市102308

出　　处：《中国分子心脏病学杂志》2016年第2期1664-1667,共4页Molecular Cardiology of China

基　　金：973项目(2014CB541601)

摘　　要：目的通过建立高血压脑出血小鼠模型,探讨血管平滑肌细胞的凋亡对高血压脑出血的作用,为预防高血压脑出血提供线索。方法选用八月龄的C57BL/6雄性小鼠,利用血管紧张素Ⅱ和L-NAME(N-硝基-L-精氨酸甲酯)构建高血压脑出血小鼠模型,小鼠处死立即取大脑组织并用HE染色进行脑出血的鉴定,分别对脑出血组和对照组的小鼠大脑进行TUNEL染色,计数血管平滑肌细胞凋亡比。结果高血压脑出血大脑血管平滑肌细胞凋亡百分比高于正常组脑血管平滑肌(6.82%vs.2.70%,P=0.002),两者比较具有统计学意义。结论血管平滑肌细胞的凋亡可能是高血压脑出血中的重要要病理改变之一。Objective This study was designed to investigate the effect of apoptosis of vascular smooth muscle cells on hypertensive cerebral hemorrhage by establishing the model of hypertensive intracerebral hemorrhage in mice,and to provide clues for the prevention of hypertensive cerebral hemorrhage.Methods In C57 BL / 6 mice,hypertensive cerebral hemorrhage was produced with angiotensin Ⅱ and L-NAME（N-nitro-L-arginine methyl ester）.The brain tissue of the mice was took immediately after it was euthanized,and cerebral hemorrhage were identified by HE staining.The mice brain of cerebral hemorrhage group and the control group was analyzed by counting apoptosis ratio of vascular smooth muscle cell through TUNEL staining.Results The percentage of apoptosis of cerebral vascular smooth muscle cells in hypertensive cerebral hemorrhage was higher than that in normal group（6.82%vs.2.70%,P=0.002）.Conclusions Apoptosis of vascular smooth muscle cells may be one of the most important pathological changes in hypertensive cerebral hemorrhage.

关 键 词：血管平滑肌 凋亡 高血压 脑出血 

分 类 号：R743.34[医药卫生—神经病学与精神病学]