高血压小鼠脑出血血管内弹性膜的病理结构变化  

Pathological Changes of Internal Elastic Membrane in Hypertensive Intracerebral Hemorrhage in Mice

作　　者：赵辉[1] 席杰[1] 田雪晴[1] 陈敬洲[1] 宋晓东[1] 

机构地区：[1]中国医学科学院北京协和医学院国家心血管病中心阜外医院心血管疾病国家重点实验室,北京市10037

出　　处：《中国分子心脏病学杂志》2016年第2期1676-1678,共3页Molecular Cardiology of China

基　　金：国家973项目(2014CB541601)

摘　　要：目的高血压脑出血是脑卒中的常见类型,尽管其发病率较缺血性卒中低,但其发病凶险,预后较差,目前对高血压条件下脑血管病理改变并导致出血知之甚少,本研究拟通过高血压脑出血小鼠模型研究高血压脑出血血管病变机制。方法将8个月的雄性C57BL/6小鼠分为两组,一组高血压脑出血组,另一组是正常对照组。在小鼠出现脑卒中行为学现象时将小鼠处死将大脑组织保存。采用HE染色确定出血位点,采用弹力纤维染色观察出血部位血管的结构。结果经弹力纤维染色发现脑出血部位血管破裂,内弹性膜出现拉直、张力消失以及内弹性膜部分缺失的现象;同时,也发现了血管壁向内破裂,导致假腔存在的现象。结论本研究发现弹性内膜的变化可能与高血压小鼠脑出血的发生相关。Objective Hypertensive intracerebral hemorrhage is a common type of stroke,although its incidence was lower than those of ischemic stroke,but its onset disaster,prognosis is poorer.At present,cerebrovascular pathological changes after hemorrhage are poorly understood.This study proposed use hypertension cerebral hemorrhage mouse model to study hypertension cerebral hemorrhage mechanism of vascular lesions.Methods Eight months male C57 BL / 6 mice were divided into hypertensive intracerebral hemorrhage group and control group.When stroke behavior occurs,the mice were sacrificed and brains were preserved.HE staining to determine the bleeding site,using elastic fibers staining to observe of vascular structures of bleeding site.Results Elastic fiber dyeing found a ruptured blood vessel hemorrhage parts,straightening and tension of internal elastic membrane were disappeared.The study also found blood vessels burst to internal,causing false lumen.Conclusion This study found that the change of elastic lining may be associated with the occurrence of hypertension cerebral hemorrhage in mice.

关 键 词：高血压脑出血 血管病变 内弹性膜 

分 类 号：R743.34[医药卫生—神经病学与精神病学]