Comparison the effects of bioaugmentation versus biostimulation on marine microbial community by PCR–DGGE： A mesocosm scale  

作　　者：Mehdi Hassanshahian Zeynab Bayat Simone Cappello Francesco Smedile Michail Yakimov 

机构地区：[1]Department of Biology, Faculty of Sciences, Shahid Bahonar University of Kerman, Kerman, Iran [2]Istituto per l'Ambiente Marino Costiero (IAMC)--CNR of Messina, Messina, Italy

出　　处：《Journal of Environmental Sciences》2016年第5期136-146,共11页环境科学学报（英文版）

基　　金：supported by the Shahid Bahonar University of Kerman and grants from EC Project “Unraveling and exploiting Mediterranean Sea microbial diversity and ecology for Xenobiotics' and pollutants' clean up” (No. ULIXES-FP7KBBE-2010-3.5-03)

摘　　要：In order to better understand the effects of biostimulation and bioaugmentation processes on a marine microbial community, three different mesocosm experiments were planned. Natural seawater（10.000 L） was artificially polluted with crude oil（1 L） and（1） inorganic nutrients（Biostimulating Mesocosm, BM）,（2） inorganic nutrients and an inoculum of Alcanivorax borkumensis SK2（Single Bioaugmentation Mesocosm, SBM）,（3） inorganic nutrients and inoculums of A. borkumensis SK2 and Thalassolituus oleivorans MIL-1（Consortium Bioaugmentation Mesocosm, CBM）. During the experimental period（20 days）, samples were taken from each mesocosm and the community structure was analyzed by PCR–DGGE. The 16 S r RNA gene DGGE banding patterns and sequence analysis demonstrated that biostimulation had the lowest effect on microbial biodiversity in the mesocosms; however, the biodiversity of the marine microbial community dramatically decreased in the CBM（Shannon index was 0.6 in T3）. The community structures among the three mesocosms were also markedly different,and major bacteria derived from DGGE bands were related to uncultured Gamma Proteobacteria. The biodegradation results show that the Single Bioaugmentation Mesocosm（SBM） system had the highest percentage of degradation（95%） in comparison to the BM mesocosm（80%） and CBM（70%）.In order to better understand the effects of biostimulation and bioaugmentation processes on a marine microbial community, three different mesocosm experiments were planned. Natural seawater（10.000 L） was artificially polluted with crude oil（1 L） and（1） inorganic nutrients（Biostimulating Mesocosm, BM）,（2） inorganic nutrients and an inoculum of Alcanivorax borkumensis SK2（Single Bioaugmentation Mesocosm, SBM）,（3） inorganic nutrients and inoculums of A. borkumensis SK2 and Thalassolituus oleivorans MIL-1（Consortium Bioaugmentation Mesocosm, CBM）. During the experimental period（20 days）, samples were taken from each mesocosm and the community structure was analyzed by PCR–DGGE. The 16 S r RNA gene DGGE banding patterns and sequence analysis demonstrated that biostimulation had the lowest effect on microbial biodiversity in the mesocosms; however, the biodiversity of the marine microbial community dramatically decreased in the CBM（Shannon index was 0.6 in T3）. The community structures among the three mesocosms were also markedly different,and major bacteria derived from DGGE bands were related to uncultured Gamma Proteobacteria. The biodegradation results show that the Single Bioaugmentation Mesocosm（SBM） system had the highest percentage of degradation（95%） in comparison to the BM mesocosm（80%） and CBM（70%）.

关 键 词：Alcanivorax Crude oil DGGE Marine environment Mesocosm 

分 类 号：X172[环境科学与工程—环境科学] X55