Purmorphamine对BV2细胞帕金森病相关基因Nurr1表达的影响  

Effect of purmorphamine on Parkinson′s disease-related Nurr1 gene expression in BV2 cells

作　　者：洪乐鹏[1] 邵帅[1] 汪光亮[1] 

机构地区：[1]广州医科大学人体解剖教研室,511436

出　　处：《中国神经免疫学和神经病学杂志》2016年第3期195-198,共4页Chinese Journal of Neuroimmunology and Neurology

基　　金：广东省科学技术厅资助项目(201301);广东省医学科研基金厅资助项目(A2013239)

摘　　要：目的探讨Purmorphamine(PM)激活小胶质细胞瘤BV2细胞中Sonic hedgehog(SHH)信号通路对帕金森病(PD)相关基因Nurr1表达的影响。方法体外培养BV2小胶质细胞并分为对照组、脂多糖(LPS)处理组、PM+LPS处理组以及PM处理组,运用荧光定量PCR(Q-PCR)检测经LPS处理后BV2细胞中SHH信号通路Smoothened(Smo)、Gli1及Nurr1基因mRNA表达情况;PM激活SHH信号通路后,Q-PCR检测Nurr1mRNA含量以及炎性反应因子白细胞介素-1β(IL-1β)、肿瘤坏死因子-α(TNF-α)的mRNA表达情况。结果(1)与对照组相比,LPS处理后4h和24h时Smo和Gli1mRNA表达均升高(P<0.01,P<0.05);(2)与对照组相比,PM处理组细胞Smo和Gli1mRNA表达升高(P<0.01),LPS组Nurr1、IL-1β、TNF-αmRNA表达亦均升高(均P<0.01);而PM+LPS组Nurr1、IL-1β、TNF-αmRNA表达均较LPS处理组下降(均P<0.01)。结论PM激活SHH信号通路能够抑制BV2细胞中Nurr1的表达,并能发挥抑制炎性反应作用。Objective To investigate the effect of Sonic hedgehog（SHH）signaling pathway activated by purmorphamine（PM）on the expression of Parkinson-related gene Nurr1 in BV2 microglial cells. Methods The routinely cultured BV2 microglial cells in vitro were divided into the control group,the lipopolysaccharide（LPS）group,the PM ＋ LPS group and the PM group.Real time quantitative PCR was used to detect contents of SHH relevant gene Smoothened（Smo）,Gli1 and Nurr1 mRNA in BV2 cell after LPS stimulation.The contents of Nurr1 and the expression of inflammatory factor-1β（IL-1β）,tumor necrosis factor-α（TNF-α）mRNA were detected by real time quantitative PCR after SHH signaling pathway activation by PM.Results（1）Compared with the control group,the expression levels of Smo and Gli1 significantly increased 4hours and24 hours after LPS stimulation（P〈0.01,P〈0.05）.（2）Compared with the control group,the expression levels of Smo and Gli1 significantly increased after PM treatment（P0.01,respectively）.The expression levels of Nurr1 and IL-1β,TNF-αalso significantly increased in the LPS group（P〈0.01,respectively）.Compared with the LPS group,the expression levels of Nurr1,IL-1βand TNF-αdecreased in the PM ＋ LPS group（P〈0.01,respectively）.Conclusions SHH signaling pathways activated by PM can inhibit the expression of Nurr1 in BV2cells,and play a role in inhibiting inflammation.

关 键 词：Purmorphamine BV2细胞 帕金森病 NURR1 炎症 

分 类 号：R741.05[医药卫生—神经病学与精神病学]