Thyroxine therapy ameliorates serum levels of eicosanoids in Chinese subclinical hypothyroidism patients  

作　　者：Yan ZHANG Bing-chang ZHANG Jin XU Meng ZHAO Zhe WANG Yong-feng SONG Hai-qing ZHANG Ling GAO Qun-ye ZHANG Jia-jun ZHAO 

机构地区：[1]Division of Endocrinology and Metabolism, Shandong Provincial Hospital affiliated with Shandong University, Ji-nan 250021, China [2]Baoshan Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai 201900, China [3]The Key Laboratory of Cardio-vascular Remodeling and Function Research, Chinese Ministry of Education and Chinese Ministry of Health The State and ShandongProvince Joint Key Laboratory of Translational Cardiovascular Medicine, Qilu Hospital of Shandong University Central Research Laboratory, The Second Hospital of Shandong University, Ji-nan 250100, China

出　　处：《Acta Pharmacologica Sinica》2016年第5期656-663,共8页中国药理学报（英文版）

摘　　要：Aim： The eicosanoids derived from phospholipids play key roles in inflammation. However, the profiles of serum eicosanoids in subclinical hypothyroidism （SH） patients and the effects of thyroxine replacement therapy （TRT） on these eicosanoids remain unclear. Many studies show that TSH regulates lipid metabolism. As eicosanoids derived from phospholipids play key roles in oxidative stress and immune function and inflammatory process, it was necessary to explore the profiles of serum eicosanoids in SH patients and the effects of thyroxine replacement therapy （TRT） on the eicosanoids. Methods： A total of 50 Chinese SH patients and 22 healthy volunteers were recruited. SH patients received TRT （L-T4, 25 and 50 mcg/d for patients with TSH〈IO.O mlU/L and TSH〉IO.O mlU/L, respectively） for 3 months. Serum levels of major eicosanoids and cPLA2 were analyzed using LC-MS and clinical biochemical assays. Results： The serum levels of cPLA2, eicosanoids （8-isoPGF2a, 11-dehydroTXB2 and 12-HETE） and 11-dehydroTXB2/6-Keto-PGF1a were significantly elevated in SH patients. The serum TSH levels were significantly correlated with the levels of cPLA2 （r=＋0.65）, 11-dehydroTXB2 （r=＋0.32） and 11-dehydroTXB2/6-Keto-PGF1a （r=＋0.37）. After 3-month TRT, the serum levels of TSH, cPLA2 and the above-mentioned eicosanoids in SH patients were significantly decreased. Conclusion： The metabolism of eicosanoids is significantly altered in Chinese SH patients, and TRT can ameliorate the abnormalities of serum eicosanoid levels.Aim： The eicosanoids derived from phospholipids play key roles in inflammation. However, the profiles of serum eicosanoids in subclinical hypothyroidism （SH） patients and the effects of thyroxine replacement therapy （TRT） on these eicosanoids remain unclear. Many studies show that TSH regulates lipid metabolism. As eicosanoids derived from phospholipids play key roles in oxidative stress and immune function and inflammatory process, it was necessary to explore the profiles of serum eicosanoids in SH patients and the effects of thyroxine replacement therapy （TRT） on the eicosanoids. Methods： A total of 50 Chinese SH patients and 22 healthy volunteers were recruited. SH patients received TRT （L-T4, 25 and 50 mcg/d for patients with TSH〈IO.O mlU/L and TSH〉IO.O mlU/L, respectively） for 3 months. Serum levels of major eicosanoids and cPLA2 were analyzed using LC-MS and clinical biochemical assays. Results： The serum levels of cPLA2, eicosanoids （8-isoPGF2a, 11-dehydroTXB2 and 12-HETE） and 11-dehydroTXB2/6-Keto-PGF1a were significantly elevated in SH patients. The serum TSH levels were significantly correlated with the levels of cPLA2 （r=＋0.65）, 11-dehydroTXB2 （r=＋0.32） and 11-dehydroTXB2/6-Keto-PGF1a （r=＋0.37）. After 3-month TRT, the serum levels of TSH, cPLA2 and the above-mentioned eicosanoids in SH patients were significantly decreased. Conclusion： The metabolism of eicosanoids is significantly altered in Chinese SH patients, and TRT can ameliorate the abnormalities of serum eicosanoid levels.

关 键 词：subclinical hypothyroidism L-T4 replacement therapy TSH CPLA2 EICOSANOIDS 11-dehydroTXB2 11-dehydroTXB2/6-Keto-PGF1a 

分 类 号：Q2[生物学—细胞生物学] S831.5[农业科学—畜牧学]